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Reducing ligation bias during small RNA library generation for next generation sequencing

Seminar
9 September 2014, 10.30am: Free
at James Hutton Institute, Dundee, Invergowrie, DD25DA
for scientists, staff and any interested parties
Tamas Dalmay

Professor Tamas Dalmay, University of East Anglia, will give this seminar titled "Reducing ligation bias during small RNA library generation for next generation sequencing" at the James Hutton Institute Dundee. This seminar will also be broadcast live to the Aberdeen site.

Abstract

Small non-coding RNAs (sRNAs) play an important role in regulating the expression of protein coding genes. Profiling sRNA expression is often the first step in understanding their biology in a given system. In the last five years next generation sequencing (NGS) of cDNA libraries of sRNAs became the standard approach of sRNA profiling. However, cDNA libraries of small RNAs for NGS are biased and we demonstrated that this is based on the ability of individual small RNAs to form stable secondary structures with the adapters. We developed an approach where adapters containing degenerated nucleotides are used for library preparation and showed that this reduces the ligation bias by allowing different sequences to form a stable structure with the pool of adapter sequences.

Biography

Tamas Dalmay graduated from the University of Budapest and obtained his PhD in molecular virology in Hungary. He moved to the UK with an EMBO fellowship in 1995 to work on the genetics of gene silencing. He is a group leader at the University of East Anglia, Norwich since 2002 where he has been working on microRNAs (miRNAs) and other non-coding short RNAs in plants and animals. He is a Professor of RNA Biology since 2012 and the Head of the School of Biological Sciences since January 2014. One of the main interests of the group is small RNA profiling and identification by next generation sequencing.

This seminar will be hosted by Dr Csaba Hornyik, Cell and Molecular Sciences.

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The James Hutton Research Institute is the result of the merger in April 2011 of MLURI and SCRI. This merger formed a new powerhouse for research into food, land use, and climate change.