Skip to navigation Skip to content

Reducing ligation bias during small RNA library generation for next generation sequencing

Important information for event attendees and external visitors

coronavirus (COVID-19)In light of the most recent advice from the UK Government about stopping non-essential travel and increasing social distancing, most of our events have been rescheduled or moved to an online format.

Our sites have been placed on a restricted access condition, which means that only staff who are doing essential work can get access. All other colleagues will be working from home or staying at home even if they are unable to work remotely.

We have excellent and free to use video conference and conference call systems and are happy to make these facilities available to help you engage with us. Meetings are taking place via video conference with participants joining individually from their own locations; check with the relevant member of staff for advice.

As the situation is constantly changing, please check the UK Government and NHS websites for the latest advice and updates.

If you have any questions or concerns, please email events@hutton.ac.uk.

Seminar
9 September 2014, 10.30am: Free
at James Hutton Institute, Dundee, Invergowrie, DD25DA
for scientists, staff and any interested parties
Tamas Dalmay

Professor Tamas Dalmay, University of East Anglia, will give this seminar titled "Reducing ligation bias during small RNA library generation for next generation sequencing" at the James Hutton Institute Dundee. This seminar will also be broadcast live to the Aberdeen site.

Abstract

Small non-coding RNAs (sRNAs) play an important role in regulating the expression of protein coding genes. Profiling sRNA expression is often the first step in understanding their biology in a given system. In the last five years next generation sequencing (NGS) of cDNA libraries of sRNAs became the standard approach of sRNA profiling. However, cDNA libraries of small RNAs for NGS are biased and we demonstrated that this is based on the ability of individual small RNAs to form stable secondary structures with the adapters. We developed an approach where adapters containing degenerated nucleotides are used for library preparation and showed that this reduces the ligation bias by allowing different sequences to form a stable structure with the pool of adapter sequences.

Biography

Tamas Dalmay graduated from the University of Budapest and obtained his PhD in molecular virology in Hungary. He moved to the UK with an EMBO fellowship in 1995 to work on the genetics of gene silencing. He is a group leader at the University of East Anglia, Norwich since 2002 where he has been working on microRNAs (miRNAs) and other non-coding short RNAs in plants and animals. He is a Professor of RNA Biology since 2012 and the Head of the School of Biological Sciences since January 2014. One of the main interests of the group is small RNA profiling and identification by next generation sequencing.

This seminar will be hosted by Dr Csaba Hornyik, Cell and Molecular Sciences.

Share our content

Share this

Tags


Printed from /events/reducing-ligation-bias-during-small-rna-library-generation-next-generation-sequencing on 13/07/20 06:55:30 AM

The James Hutton Research Institute is the result of the merger in April 2011 of MLURI and SCRI. This merger formed a new powerhouse for research into food, land use, and climate change.