Edgar Huitema
Current research interests
My current research is aimed at understanding the mechanisms by which Phytophthora sp., cause epidemics on crop plants. More specifically, work in my group focuses on the functions of the Crinklers, a novel class of cytoplasmic effectors from Phytophthora. In addition, we are interested in the events that couple signaling cues to developmental transitions during pathogen ingress. We hypothesise that these processes are critical for disease establishment and form a possible basis for regulation of effector secretion and delivery.
Collaborators
Professor Paul Birch - Division of Plant Sciences, University of Dundee at The James Hutton Institute, Dundee
Professor Kurt H. Lamour - Department of Entomology and Plant Pathology, University of Tenessee
Professor Sophien Kamoun - The Sainsbury Laboratory, Norwich
Recent Publications
Bibliography
- Stam, R.; Motion, G.B.; Martinez-Heredia, V.; Boevink, P.C.; Huitema, E. (2021) A conserved oomycete CRN effector targets tomatoTCP14-2 to enhance virulence., Molecular Plant-Microbe Interactions, 34(3), 309-318
- Thilliez, G.J.A.; Armstrong, M.R.; Lim, T.Y.; Baker, K.; Jouet, A.; Ward, B.; van Oosterhout, C.; Jones, J.D.G.; Huitema, E.; Birch, P.R.J.; Hein, I. (2019) Pathogen enrichment sequencing (PenSeq) enables population genomic studies in oomycetes., New Phytologist, 221, 1634-1648.
- Pham, J.; Stam, R.; Heredia, V.M.; Csukai, M.; Huitema, E. (2018) An NMRA-like protein regulates gene expression in Phytophthora capsici to drive the infection cycle on tomato., Molecular Plant-Microbe Interactions, 31, 665-677.
- Amaro, T.M.M.M.; Thilliez, G.J.A.; McLeod, R.A.; Huitema, E. (2018) Random mutagenesis screen shows that Phytophthora capsici CRN83_152-mediated cell death is not required for its virulence function(s)., Molecular Plant Pathology, 19, 1114-1126.
- Cummins, M.; Huitema, E. (2017) Effector-decoy pairs: another countermeasure emerging during host-microbe co-evolutionary arms races?, Molecular Plant, 10, 662-664.
- Howden, A.J.M.; Stam, R.; Heredia, V.M.; Motion, G.B.; ten Have, S.; Hodge, K.; Amaro, T,M.M.M.; Huitema, E. (2017) Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity., New Phytologist, 215, 309-322.
- Amaro, T.M.M.M.; Thilliez, G.J.A.; Motion, G.B.; Huitema, E. (2017) A perspective on crn proteins in the genomics age: evolution, classification, delivery and function revisited., Frontiers in Plant Science, 8, Article No. 99.
- Motion, G.B.; Howden, A.J.M.; Huitema, E.; Jones, S. (2015) DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool., Nucleic Acids Research, 43, Article No.e158.
- Motion, G.B.; Amaro, T.M.M.M.; Kulagina, N.; Huitema, E. (2015) Nuclear processes associated with plant immunity and pathogen susceptibility., Briefings in Functional Genomics, 14, 243-252.
- Stam, R.; Howden, A.J.M.; Delgado-Cerezo, M.; Amaro, T.M.M.M.; Motion, G.B.; Pham, J.; Huitema, E. (2013) Characterization of cell death inducing Phytophthora capsici CRN effectors suggests diverse activities in the host nucleus., Frontiers in Plant Science, 4, 387.
- Stam, R.; Jupe, J.; Howden, A.J.M.; Morris, J.A.; Boevink, P.C.; Hedley, P.E.; Huitema, E. (2013) Identification and characterisation CRN effectors in Phytophthora capsici shows modularity and functional diversity., PLoS ONE, 8, Article No. e59517.
- Jupe, J.; Stam R.; Howden A.J.M.; Morris J.A.; Zhang R.; Hedley P.E.; Huitema E. (2013) Phytophthora capsici-tomato interaction features dramatic shifts in gene expression associated with a hemi-biotrophic lifestyle., Genome Biology, 14, Article No. R63.
- Lamour, K.H.; Stam, R.; Jupe, J.; Huitema, E. (2012) The oomycete broad-host-range pathogen Phytophthora capsici., Molecular Plant Pathology, 13, 329-337.
- Howden, A.J.M.; Huitema. E. (2012) Effector-triggered post-translational modifications and their role in suppression of plant immunity., Frontiers in Plant Science, 3, 160.
- Lamour, K.H.; Mudge, J.; Gobena, D.; Hurtado-Gonzales, O.P.; Schmutz, J.; Kuo, A.; Miller, N.A.; Rice, B.J.; Raffaele, S.; Cano, L.M.; Bharti, A.K.; Donahoo, R.S.; Finley, S.; Huitema, E.; Hulvey, J.; Platt, D.; Salamov, A.; Savidor, A.; Sharma, R.; Stam, R.; Storey, D.; Thines, M.; Win, J.; Haas, B.J.; Dinwiddie, D.L.; Jenkins, J.; Knight, J.R.; Affourtit, J.P.; Han, C.S.; Chertkov, O.; Lindquist, E.A.; Detter, C.; Grigoriev, I.V.; Kamoun, S.; Kingsmore, S.F. (2012) Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen phytophthora capsici., Molecular Plant-Microbe Interactions, 25, 1350-1360.
- Huitema, E.; Smoker, M.; Kamoun, S. (2011) A straightforward protocol for electro-transformation of Phytophthora capsici zoospores., In: McDowall, J.M. (ed.). Plant Immunity: Methods and Protocols. Humana Press, Totowa, New Jersey, USA, pp129-136.
- Schornack, S.; Van Damme, M.; Bozkurt, T.O.; Cano, L.M.; Smoker, M.; Thines, M.; Gaulin, E.; Kamoun, S.; Huitema, E. (2010) Ancient class of translocated oomycete effectors targets the host nucleus., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 17421-17426.
- Oliva, R.; Win, J.; Raffaele, S.; Boutemy, L.; Bozkurt, T.O.; Chaparro-Garcia, A.; Segretin, M.E.; Stam, R.; Schornack, S.; Cano, L.M.; Van Damme, M.; Huitema, E.; Thines, M.; Banfield, M.J.; Kamoun, S. (2010) Recent developments in effector biology of filamentous plant pathogens., Cellular Microbiology, 12, 705-715.
- Bollmann, S.R.; Bos, J.I.B.; Bulone, V.; Cai, G.H.; Cakir, C.; Carrington, J.C.; Chawner, M.; Conti, L; Costanzo, S.; Ewan, R.; Fahlgren, N.; Fischbach, M.A.; Fugelstad, J.; Gilroy, E.M.; Gnerre, S.; Green, P.J.; Grenville-Briggs, L.J.; Griffith, J.; Grunwald, N.J.; Horn, K.; Horner, N.R.; Hu, C.H.; Huitema, E.; Jeong, D.H.; Jones, A.M.E.; Jones, J.D.G.; Jones, R.W.; Karlsson, E.K.; Kunjeti, S.G.; Lamour, K.; Liu, Z.Y.; Ma, L.J.; MacLean, D.; Chibucos, M.C.; McDonald, H.; McWalters, J.; Meijer, H.J.G.; Morgan, W.; Morris, P.F.; Munro, C.A.; O'Neill, K.; Ospina-Giraldo, M.; Pinzon, A.; Pritchard, L.; Ramsahoye, B.; Ren, Q.H.; Restrepo, S.; Roy, S.; Sadanandom, A.; Savidor, A.; Schornack, S.; Schwartz, D.C.; Schumann, U.D.; Schwessinger, B.; Seyer, L.; Sharpe, T.; Silvar, C.; Song, J.; Studholme, D.J.; Sykes, S.; Thines, M.; van de Vondervoort, P.J.I.; Phuntumart, V.; Wawra, S.; Weide, R.; Win, J.; Young, C.; Zhou, S.G.; Fry, W.; Meyers, B.C.; van West, P.; Ristaino, J.; Govers, F.; Birch, P.R.J.; Whisson, S.C.; Judelson, H.S.; Nusbaum, C. (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans., Nature, 461, 393-398.
- Bos, J.I.B.; Kanneganti, T.; Young, C.; Cakir, C.; Huitema, E.; Win, J.; Armstrong, M.R.; Birch, P.R.J.; Kamoun, S. (2006) The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana., Plant Journal, 48, 165-176.
- Randall, T.A.; Dwyer, R.A.; Huitema, E.; Beyer, K.; Cvitanich, C.; Kelkar, H.; Ah, Fong, AM.V.; Gates, K.; Roberts, S.; Yatzkan, E.; Gaffney, T.; Law, M.;Testa, A.; Torto-Alalibo, T.; Zhang, M.; Zheng, L.; Mueller, E.; Windass, J.; Binder, A.; Birch, P.R.J.; Gisi, U.; Govers, F.; Gow, N.A.; Mauch, F.; West, P.V.; Waugh, M.E.; Yu, J.; Boller, T.; Kamoun, S.; Lam, S.T.; Judelson, H.S. (2005) Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi., Molecular Plant-Microbe Interactions, 18, 229-243.
- Stam, R.; Motion, G.B.; Martinez-Heredia, V.; Boevink, P.C.; Huitema, E. (2021) A conserved oomycete CRN effector targets tomatoTCP14-2 to enhance virulence., Molecular Plant-Microbe Interactions, 34(3), 309-318
- Thilliez, G.J.A.; Armstrong, M.R.; Lim, T.Y.; Baker, K.; Jouet, A.; Ward, B.; van Oosterhout, C.; Jones, J.D.G.; Huitema, E.; Birch, P.R.J.; Hein, I. (2019) Pathogen enrichment sequencing (PenSeq) enables population genomic studies in oomycetes., New Phytologist, 221, 1634-1648.
- Pham, J.; Stam, R.; Heredia, V.M.; Csukai, M.; Huitema, E. (2018) An NMRA-like protein regulates gene expression in Phytophthora capsici to drive the infection cycle on tomato., Molecular Plant-Microbe Interactions, 31, 665-677.
- Amaro, T.M.M.M.; Thilliez, G.J.A.; McLeod, R.A.; Huitema, E. (2018) Random mutagenesis screen shows that Phytophthora capsici CRN83_152-mediated cell death is not required for its virulence function(s)., Molecular Plant Pathology, 19, 1114-1126.
- Cummins, M.; Huitema, E. (2017) Effector-decoy pairs: another countermeasure emerging during host-microbe co-evolutionary arms races?, Molecular Plant, 10, 662-664.
- Howden, A.J.M.; Stam, R.; Heredia, V.M.; Motion, G.B.; ten Have, S.; Hodge, K.; Amaro, T,M.M.M.; Huitema, E. (2017) Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity., New Phytologist, 215, 309-322.
- Amaro, T.M.M.M.; Thilliez, G.J.A.; Motion, G.B.; Huitema, E. (2017) A perspective on crn proteins in the genomics age: evolution, classification, delivery and function revisited., Frontiers in Plant Science, 8, Article No. 99.
- Motion, G.B.; Howden, A.J.M.; Huitema, E.; Jones, S. (2015) DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool., Nucleic Acids Research, 43, Article No.e158.
- Motion, G.B.; Amaro, T.M.M.M.; Kulagina, N.; Huitema, E. (2015) Nuclear processes associated with plant immunity and pathogen susceptibility., Briefings in Functional Genomics, 14, 243-252.
- Stam, R.; Howden, A.J.M.; Delgado-Cerezo, M.; Amaro, T.M.M.M.; Motion, G.B.; Pham, J.; Huitema, E. (2013) Characterization of cell death inducing Phytophthora capsici CRN effectors suggests diverse activities in the host nucleus., Frontiers in Plant Science, 4, 387.
- Stam, R.; Jupe, J.; Howden, A.J.M.; Morris, J.A.; Boevink, P.C.; Hedley, P.E.; Huitema, E. (2013) Identification and characterisation CRN effectors in Phytophthora capsici shows modularity and functional diversity., PLoS ONE, 8, Article No. e59517.
- Jupe, J.; Stam R.; Howden A.J.M.; Morris J.A.; Zhang R.; Hedley P.E.; Huitema E. (2013) Phytophthora capsici-tomato interaction features dramatic shifts in gene expression associated with a hemi-biotrophic lifestyle., Genome Biology, 14, Article No. R63.
- Lamour, K.H.; Stam, R.; Jupe, J.; Huitema, E. (2012) The oomycete broad-host-range pathogen Phytophthora capsici., Molecular Plant Pathology, 13, 329-337.
- Howden, A.J.M.; Huitema. E. (2012) Effector-triggered post-translational modifications and their role in suppression of plant immunity., Frontiers in Plant Science, 3, 160.
- Lamour, K.H.; Mudge, J.; Gobena, D.; Hurtado-Gonzales, O.P.; Schmutz, J.; Kuo, A.; Miller, N.A.; Rice, B.J.; Raffaele, S.; Cano, L.M.; Bharti, A.K.; Donahoo, R.S.; Finley, S.; Huitema, E.; Hulvey, J.; Platt, D.; Salamov, A.; Savidor, A.; Sharma, R.; Stam, R.; Storey, D.; Thines, M.; Win, J.; Haas, B.J.; Dinwiddie, D.L.; Jenkins, J.; Knight, J.R.; Affourtit, J.P.; Han, C.S.; Chertkov, O.; Lindquist, E.A.; Detter, C.; Grigoriev, I.V.; Kamoun, S.; Kingsmore, S.F. (2012) Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen phytophthora capsici., Molecular Plant-Microbe Interactions, 25, 1350-1360.
- Schornack, S.; Van Damme, M.; Bozkurt, T.O.; Cano, L.M.; Smoker, M.; Thines, M.; Gaulin, E.; Kamoun, S.; Huitema, E. (2010) Ancient class of translocated oomycete effectors targets the host nucleus., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 17421-17426.
- Oliva, R.; Win, J.; Raffaele, S.; Boutemy, L.; Bozkurt, T.O.; Chaparro-Garcia, A.; Segretin, M.E.; Stam, R.; Schornack, S.; Cano, L.M.; Van Damme, M.; Huitema, E.; Thines, M.; Banfield, M.J.; Kamoun, S. (2010) Recent developments in effector biology of filamentous plant pathogens., Cellular Microbiology, 12, 705-715.
- Bollmann, S.R.; Bos, J.I.B.; Bulone, V.; Cai, G.H.; Cakir, C.; Carrington, J.C.; Chawner, M.; Conti, L; Costanzo, S.; Ewan, R.; Fahlgren, N.; Fischbach, M.A.; Fugelstad, J.; Gilroy, E.M.; Gnerre, S.; Green, P.J.; Grenville-Briggs, L.J.; Griffith, J.; Grunwald, N.J.; Horn, K.; Horner, N.R.; Hu, C.H.; Huitema, E.; Jeong, D.H.; Jones, A.M.E.; Jones, J.D.G.; Jones, R.W.; Karlsson, E.K.; Kunjeti, S.G.; Lamour, K.; Liu, Z.Y.; Ma, L.J.; MacLean, D.; Chibucos, M.C.; McDonald, H.; McWalters, J.; Meijer, H.J.G.; Morgan, W.; Morris, P.F.; Munro, C.A.; O'Neill, K.; Ospina-Giraldo, M.; Pinzon, A.; Pritchard, L.; Ramsahoye, B.; Ren, Q.H.; Restrepo, S.; Roy, S.; Sadanandom, A.; Savidor, A.; Schornack, S.; Schwartz, D.C.; Schumann, U.D.; Schwessinger, B.; Seyer, L.; Sharpe, T.; Silvar, C.; Song, J.; Studholme, D.J.; Sykes, S.; Thines, M.; van de Vondervoort, P.J.I.; Phuntumart, V.; Wawra, S.; Weide, R.; Win, J.; Young, C.; Zhou, S.G.; Fry, W.; Meyers, B.C.; van West, P.; Ristaino, J.; Govers, F.; Birch, P.R.J.; Whisson, S.C.; Judelson, H.S.; Nusbaum, C. (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans., Nature, 461, 393-398.
- Bos, J.I.B.; Kanneganti, T.; Young, C.; Cakir, C.; Huitema, E.; Win, J.; Armstrong, M.R.; Birch, P.R.J.; Kamoun, S. (2006) The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana., Plant Journal, 48, 165-176.
- Randall, T.A.; Dwyer, R.A.; Huitema, E.; Beyer, K.; Cvitanich, C.; Kelkar, H.; Ah, Fong, AM.V.; Gates, K.; Roberts, S.; Yatzkan, E.; Gaffney, T.; Law, M.;Testa, A.; Torto-Alalibo, T.; Zhang, M.; Zheng, L.; Mueller, E.; Windass, J.; Binder, A.; Birch, P.R.J.; Gisi, U.; Govers, F.; Gow, N.A.; Mauch, F.; West, P.V.; Waugh, M.E.; Yu, J.; Boller, T.; Kamoun, S.; Lam, S.T.; Judelson, H.S. (2005) Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi., Molecular Plant-Microbe Interactions, 18, 229-243.
- Huitema, E.; Smoker, M.; Kamoun, S. (2011) A straightforward protocol for electro-transformation of Phytophthora capsici zoospores., In: McDowall, J.M. (ed.). Plant Immunity: Methods and Protocols. Humana Press, Totowa, New Jersey, USA, pp129-136.
