Genotyping
To determine the genetic basis for the phenotypic traits of an organism, it is essential to identify the underlying genotype. Although low-throughput assays, such as Simple Sequence Repeats (SSRs), continue to be used and processed at The James Hutton Institute, Single Nucleotide Polymorphisms (SNPs) enable transition to high-throughput multiplexed genotyping.
We have also recently acquired a digital PCR (Qiagen QIAcuity) system which enables genotyping, and also highly sensitive mutation detection, gene expression analysis and copy number variation.
Please contact Pete Hedley or Clare Macaulay for further information and available options, including pricing.
'Plant to Genotype' pipeline
The facility has recently developed a high throughput ‘Plant to Genotype’ pipeline, enabling researchers to submit samples as leaf material, saving them time and resources. Genomic DNA is extracted using Qiagen robotics and the high-quality gDNA checked and quantified using Picogreen technology. Currently, high-throughput barley genotyping is processed using a custom-designed Illumina iSelect chip, representing 50,000 gene variants. The pipeline is currently being adapted for other plant species.
