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Next generation sequencing

Illumina MiSeq sequencer
Illumina MiSeq sequencer

Illumina

Short-read next generation sequencing (NGS) was adopted by the Genomics facility in 2012, with acquisition of an Illumina MiSeq which can generate up to 25 M paired-end reads of 100-300 bp. Recently, we have also installed a NextSeq 2000, which has much higher output of up to 1.2 BN paired-end reads.

We support processing of a vast array of small to medium sized NGS projects within the facility, including small whole-genome shotgun (WGS) sequencing, transcriptomics (RNAseq), metabarcoding (16S & ITS), whole genome metagenomics and target enrichment sequencing. For larger projects, we can recommend external service providers.

 

 

Oxford Nanopore Technologies

Long-read sequencing is supported using Oxford Nanopore Technologies (ONT) equipment. We currently have a MinION available and will acquire a new high-output PromethION P2 in November 2023. This will enable generation of a large volume (100 Gb) of long-read data, with potential for ultralong megabase reads.

ONT will complement short-read data to allow whole genome scaffolding, native RNA sequencing, detection of natural base modifications and on-the-fly sequencing selection (adaptive sampling).

 

Please contact Pete Hedley to discuss potential NGS projects and costs.

 

 

 

Research

Areas of Interest


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The James Hutton Research Institute is the result of the merger in April 2011 of MLURI and SCRI. This merger formed a new powerhouse for research into food, land use, and climate change.