Linda Milne

Bioinformatics Specialist
Information and Computational Sciences
T: +44 (0)344 928 5428
  • Genome assembly, SNP discovery, marker/microarray design, genome annotation.
  • Development of visualization and analysis tools for sequence and genetic data.
  • Database and information management systems for Institute research projects.
  • Automated data pipelines for producing good quality, contaminant-free DNA sequences, contig assemblies and sequence annotation.

Publications

The following Publications have not yet been migrated to the James Hutton Institute's Pure service and relate to the research outputs from the two legacy organisations: The Macaulay Land Use Research Institute and The Scottish Crop Research Institute.

Journals

Technical / contract reports

  • Milne, I.; Shaw, P.; Milne, L.; Bayer, M.; Stephen, G.; Marshall, D.F. (2010) Visualising genetic diversity., Annual Report of the Scottish Crop Research Institute for 2009, pp21-22.
  • Ramsay, L.; Hedley, P.E.; Liu, H.; McCallum, N.; Mudie, S.; Cardle, L.; Harrower, B.E.; Machray, G.C.; Marshall, D.F.; Waugh, R. (2002) Barley transcriptome resources., Annual Report of the Scottish Crop Research Institute for 2001/2002, pp151-152.
  • Cardle, L.; Macaulay, M.; Marshall, D.F.; Milbourne, D.; Ramsay, L.; Waugh, R. (2000) SSR frequency and occurrence in plant genomes., Annual Report of the Scottish Crop Research Institute for 1999/2000, pp108-110.

Conference papers

  • Bayer, M.M.; Russell, J.R.; Milne, L.; Hedley, P.E.; Marshall, D.F. (2010) SNP discovery in barley Illumina data using Mosaik, GigaBayes and Tablet., 2nd Workshop of COST StatSeq Action (TD0801), Ghent, Belgium, 20-21 May 2010.
  • Shaw, P.; Cardle, L.; Milne, I.; Waugh, R.; Flavell, A.; Thomas, W.T.B.; Grzebyta, J.; Bayer, M.; Marshall, D.F. (2009) Germinate 2: A platform for Illumina SNP genotyping experiments and high volume phenotypic data., Plant & Animal Genome XVII, San Diego, California, 10-14 January 2009.
  • Rostoks, N.; Mudie, S.; Bonar, N.; Cardle, L.; Marshall, D.F.; Waugh, R. (2007) SNP and haplotype diversity in coding and non-coding regions of European spring and winter barley., II International Vavilov Conference Crop Genetic Resources in the 21st Century, St Petersburg, Russia, 26-30 November 2007.
  • Rostoks, N.; Mudie, S.; Bonar, N.; Cardle, L.; Marshall, D.F.; Waugh, R. (2005) Single nucleotide polymorphism discovery and mapping in barley., Plant & Animal Genome Conference (PAG) XIII, San Diego, California, 15-19 January 2005.
  • Marshall, D.F.; Shaw, P.; Cardle, L. (2005) The plant genomics database infrastructure at the Scottish Crop Research Institute., Plant & Animal Genome XII, San Diego, California, 15-19 January 2005 (Abstract).
  • Rostoks, N.; Cardle, L.; Mudie, S.; Svensson, J.T.; Walia, H.; Rodriguez, E.M.; Wanamaker, S.; Hedley, P.E.; Liu, H.; Ramsay, L.; Russell, J.R.; Close, T.J.; Marshall, D.F.; Waugh, R. (2004) Single nucleotide polymorphism mapping of the barley genes involved in abiotic stresses., Barley Genetics IX, Proceedings of 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June 2004.
  • Russell, J.R.; Ramsay, L.; Booth, A.; Macaulay, M.; Cardle, L.; Marshall, D.F.; Powell, W.; Waugh, R. (2004) Development of gene based molecular markers in barley., Barley Genetics IX, Proceedings of 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June 2004.
  • Ibrahim, A.F.M.; Hedley, P.E.; Cardle, L.; Marshall, D.F.; Waugh, R. (2004) Analysis of patterns of gene expression during barley grain development using Serial Analysis of Gene Expression (SAGE)., Barley Genetics IX, Proceedings of the 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June 2004.
  • Ibrahim, A.F.M.; Hedley, P.E.; Cardle, L.; Kruger, W.; Marshall, D.F.; Muehlbauer, G.J.; Waugh, R. (2004) A comparative study of sage and microarray analysis in transcript profiling in barley., Plant & Animal Genome XII, San Diego, 10-14 January 2004, 120.
  • McNicoll, J.; Tierney, I.; Milbourne, D.; Isidore, E.; McLean, K.; Williamson, S.; Cardle, L.; Purvis, A.; Waugh, R.; Bryan, G.J. (2003) Progress towards construction of a targeted physical map of a region of potato chromosome V., Plant and Animal Genome XI, San Diego, USA, 13-17January 2003.
  • Taleb, N.; Avrova, A.O.; Stewart, H.E.; Cardle, L.; Jauffeerally-Fakim, Y.; Birch, P.R.J. (2002) Unravelling the molecular mechanisms of resistance in potato to Phytophthora infestans the causal agent of late blight., BSSP Presidential Conference: Plant Pathology and Global Food Security, Imperial College, London, 8-10 July 2002.
  • Rokka, V.; Avrova, A.O.; Hein, I.; Gilroy, E.M.; Cardle, L.; Birch, P.R.J. (2002) Unravelling signalling processes in the potato R gene resistance response to Phytophthora infestans., GILB GILB’02 conference – Late Blight: Managing the Global Threat, Hamburg, Germany, 11-13 July 2002, 152.
  • Woodhead, M.; Russell, J.R.; Squirrell, J.; Hollingsworth, P.M.; Cardle, L.; Ramsay, L.; Gibby, M.; Powell, W. (2002) The application of EST-derived SSRs in plant conservation genetics., Plant Conservation in Scotland Conference, September 2002.
  • Woodhead, M.; Russell, J.R.; Ramsay, L.; Cardle, L.; Powell, W. (2002) The development and utility of SSRs derived from expressed sequence tags. Poster., Ecological Genetics Group Meeting.
  • Marshall, D.F.; Cardle, L.; Ramsay, L.; Biscoe, P.V. (2001) Barley and potato bioinformatics at SCRI., Proceedings of Plant and Animal Genome, p673.
  • Bell, K.S.; Avrova, A.O.; Holeva, M.C.; Cardle, L.; Morris, W.L.; De Jong, W.; Toth, I.K.; Waugh, R.; Bryan, G.J.; Birch, P.R.J. (2001) Sample sequencing of large, overlapping DNA fragments from Erwinia carotovora subsp. atroseptica reveals novel putative phytopathogenicity genes and allows comparison with the model animal pathogen Escherichia coli., Agricultural Microbes Genomes II. San Diego, USA, 17-19 January 2001.
  • May, S.; Bradnam, K.; Cardle, L.; Cartinhour, S.; Couchman, M.; Davenport, G.; Devos, K.; Dicks, J.; Gale, M.; Huang, L.; Marshall, D.F.; McWilliam, H.; Ougham, H.; Trick, M.; Waugh, R. (2000) UK CropNet 99 – the second phase., Journal of Experimental Botany, 51, 22-23.
  • Waugh, R.; Ramsay, L.; Macaulay, M.; McLean, K.; Thomas, W.T.B.; Ellis, R.P.; Swanston, J.S.; Russell, J.R.; Cardle, L.; Harrower, B.E.; Hedley, P.E.; Powell, W.; Machray, G.C. (1999) Identification and improvement of components of barley malting quality., Proceedings of the 9th Australian Barley Technical Symposium, Melbourne, 12-16 September 1999, pp98-112.
  • Cardle, L.; Waugh, R.; Marshall, D.F. (1998) UK CROPNET Barley Db., BBSRC PAGA/GAIT Workshop, Edinburgh, April 1998.

Conference posters / abstracts

  • Shaw, P.; Milne, I.; Cardle, L.; Waugh, R.; Flavell, A.J.; Stephen, G.; Bayer, M.; Graham, M.; Kennedy, J.; Marshall, D.F. (2011) Germinate 2 – Storage, visualization and analysis of high volume genotypic, phenotypic and pedigree data., Plant and Animal Genome XIX, San Diego, California, USA, 15-19 January 2011. (Poster)
  • Flavell, A.J.; Syed, N.H.; Ho, T.; Cardle, L.; Xu, X.; Bayer, M.M.; Marshall, D.F. (2011) Genome-Tagged Amplification (GTA) – A PCR-based method to prepare sample-tagged amplicons from hundreds of individuals for next generation sequencing., Plant and Animal Genome XIX, San Diego, California, USA, 15-19 January 2011. (Poster)
  • Simpson, C.G.; Brown, J.W.S.; Barta, A.; McNicol, J.W.; Maronova, M.; Kalyna, M.; Kusenda, B.; Milne, L. (2011) SR-protein specific changes in Arabidopsis alternative splicing., Annual Main Meeting of Society for Experimental Biology, Glasgow, 1-4 July 2011. (Poster)
  • Simpson, C.G.; Kalyna, M.; Lewandowska, D.; Kusenda, B.; Fuller, J.; Milne, L.; McNicol, J.W.; Clark, G.; Barta, A.; Brown, J.W.S. (2010) Alternative splicing and NMD in Arabidopsis., EURASNET Annual Meeting 2010, Lisbon, Portugal, 1-4 March 2010 (Poster).
  • Simpson, C.G.; Kalyna, M.; Lewandowska, D.; Kusenda, B.; Fuller, J.; Milne, L.; McNicol, J.W.; Barta, A.; Brown, J.W.S. (2010) Alternative splicing and nonsense mediated decay in Arabidopsis., Biochemical Society: Post-Transcriptional Control: mRNA Translation, Localisation and Turnover, Edinburgh, 8-10 June 2010. 227 (Poster).
  • Simpson, C.G.; Kalyna, M.; Lewandowska, D.; Kusenda, B.; Fuller, J.; Milne, L.; McNicol, J.W.; Clark, G.; Barta, A.; Brown, J.W.S. (2010) Alternative splicing and NMD in Arabidopsis., RNA 2010, Seattle, WA, USA, 22-26 June 2010. 193 (Poster).
  • Marshall, D.F.; Milne, I.; Bayer, M.; Shaw, P.; Cardle, L.; Stephen, G.; Ramsay, L.; Comadran, J.; Thomas, W.T.B.; Russell, J.R.; Flavell, A.J.; Waugh, R. (2010) What you see is what you get: SCRI visualization tools for SNP discovery, genotype analysis and comparative mapping., Plant & Animal Genome XVIII, San Diego, California, USA, 9-13 January 2010 (Abstract).
  • Marshall, D.F.; Milne, I.; Bayer, M.; Shaw, P.; Cardle, L.; Stephen, G.; Ramsay, L.; Comadran, J.; Thomas, W.T.B.; Russell, J.R.; Flavell, A.J.; Waugh, R. (2010) Tablet, flapjack and strudel; SCRI visualization tools to support SNP discovery, genotype analysis and comparative mapping., Plant & Animal Genome XVIII, San Diego, California, USA, 9-13 January 2010 (Poster).
  • Shaw, P.; Cardle, L.; Milne, I.; Waugh, R.; Flavell, A.J.; Stephen, G.; Bayer, M.M.; Graham, M.; Kennedy, J.; Marshall, D.F. (2010) Germinate 2 – storage, visualization and analysis of high volume genotypic, phenotypic and pedigree data., Plant & Animal Genome XVIII, San Diego, California, USA, 9-13 January 2010 (Abstract).
  • Simpson, C.G.; Kalyna, M.; Lewandowska, D.; Kusenda, B.; Fuller, J.; Cardle, L.; McNicol, J.W.; Clark, G.; Barta, A.; Brown, J.W.S. (2009) Alternative splicing and NMD in Arabidopsis., RNA 2009, Madison, WI, USA, 26-31 May 2009. (Poster)
  • Simpson, C.G.; Kalyna, M.; Lewandowska, D.; Kusenda, B.; Fuller, J.; Cardle, L.; McNicol, J.W.; Clark, G.; Barta, A.; Brown, J.W.S. (2009) Alternative splicing and NMD in Arabidopsis., 20th International Conference on Arabidopsis Research, Edinburgh, 30 June – 4 July, 2009 (Poster).
  • Simpson, C.G.; Kalyna, M.; Lewandowska, D.; Kusenda, B.; Fuller, J.; Cardle, L.; McNicol, J.W.; Clark, G.; Barta, A.; Brown, J.W.S. (2009) Alternative splicing and NMD in Arabidopsis., 4th Annual EURASNET Meeting, Assisi, Italy, 23-24 April 2009 (Poster).
  • Shaw, P.; Rostoks, N.; Leader, D.; Waugh, R.; Cardle, L.; Marshall, D.F. (2006) Barley mutant and SNP databases at SCRI., Plant & Animal Genome XIII, San Diego, California, USA, 14-18 January 2006 (Abstract).
  • Cardle, L.; Shaw, P.; Brown, J.W.S.; Oparka, K.J.; Marshall, D.F. (2004) The development of an integrated suite of plant databases at the SCRI., Plant & Animal Genome XI, San Diego, California, USA, 10-14 January 2004 (Abstract).
  • Rostoks, N.; Cardle, L.; Mudie, S.; Svensson, J.T.; Walia, H.; Rodriguez, E.M.; Wanamaker, S.; Hedley, P.E.; Liu, H.; Ramsay, L.; Russell, J.R.; Close, T.J.; Marshall, D.F.; Waugh, R. (2004) Single nucleotide polymorphism mapping of the barley genes involved in abiotic stresses., Czech Journal of Genetics and Plant Breeding, 40, 52.
  • Rostoks, N.; Cardle, L.; Mudie, S.; Ramsay, L.; Hedley, P.E.; Russell, J.R.; Caldwell, D.; Marshall, D.F.; Waugh, R. (2004) Single nucleotide polymorphism discovery in barley genes differentially expressed during abiotic stresses., Plant & Animal Genome XII, San Diego, 10-14 January 2004, 146.
  • Marshall, D.F.; Ramsay, L.; Cardle, L.; Russell, J.R.; Hedley, P.E.; Booth, A.; Fuller, J.D.; Machray, G.C.; Waugh, R. (2001) Electronic single nucleotide polymorphism in barley EST’s: Discovery, Validation and Mapping., Abstracts of Plant, Animal & Microbe Genomes X Conference, San Diego, California, January 2001.

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