Malcolm Macaulay

Research

• Studying patterns of genetic variation shown by elite barley cultivars and the use of this information to gain insights into the control of characters of economic, agronomic, and environmental importance.
• Characterisation of agronomically important genes.
• High throughput SNP genotyping using Illumina Infinium 50K assay and associated analysis.
• Supervision, training, and management associated with Genome Technology
• Support for Genotyping at The James Hutton Institute, including troubleshooting software, hardware, and technical issues.
• STEM partner

Past research

• Biochemical and molecular marker development and utilisation in barley.

Publications

Journals

• Arrieta Salgado, M.; Macaulay, M.; Colas, I.; Schreiber, M.; Shaw, P.; Waugh, R.; Ramsay, L. (2021) An Induced Mutation in HvRECQL4 Increases the Overall Recombination and Restores Fertility in a Barley HvMLH3 Mutant Background, Frontiers in Plant Science, 12, Art. 706560
• Barakate, A.; Arrieta, M.; Macaulay, M.; Vivera, S.; Davidson, D.; Stephens, J.; Orr, J.; Schreiber, M.; Ramsay, L.; Halpin, C.; Waugh, R. (2021) Downregulation of barley Regulator of Telomere Elongation Helicase 1 alters the distribution of meiotic crossovers., Frontiers in plant science, 12, Article No. 745070
• Barakate, A.; Orr, J.; Schreiber, M.; Colas, I.; Lewandowska, D.; McCallum, N.; Macaulay, M.; Morris, J.; Arrieta, M.; Hedley, P.E.; Ramsay, L.; Waugh, R. (2021) Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I., Frontiers in Plant Science, 11.
• Looseley, M.E.; Ramsay, L.; Bull, H.; Swanston, J.S.; Shaw, P.D.; Macaulay, M.; Booth, A.; Russell, J.R.; Waugh, R.; Thomas, W.T.B. (2020) Association mapping of malting quality traits in UK spring and winter barley cultivar collections, Theoretical and Applied Genetics, 133, 2567-2582.
• Houston, K.; Qiu, J.; Wege, S.; Hrmova, M.; Oakey, H.; Qu, Y.; Smith, P.; Situmorang, A.; Macaulay, M.; Flis, P.; Bayer, M.; Roy, S.; Halpin, C.; Russell, J.; Schreiber, M.; Byrt, C.; Gilliham, M.; Salt, D.E.; Waugh, R. (2020) Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5., Communications Biology, 3, Article No. 258.
• Orman-Ligeza, B.; Harwood, W.; Hedley, P.E.; Hinchcliffe, A.; Macaulay, M.; Uauy, C.; Trafford, K. (2020) TRA1: A locus responsible for controlling agrobacterium-mediated transformability in barley., Frontiers in Plant Science, 11, Article No. 355.
• Macaulay, M.; Ramsay, L.; Ahman, I. (2020) Quantitative trait locus for resistance to the aphid Rhopalosiphum padi L. in barley (Hordeum vulgare L.) is not linked with a genomic region for gramine concentration., Arthropod-Plant Interactions, 14, 57-65.
• Schreiber, M.; Barakate, A.; Uzrek, N.; Macaulay, M.; Sourdille, A.; Morris, J.; Hedley, P.E.; Ramsay, L.; Waugh, R. (2019) A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing., Plant Methods, 15, Article No. 99.
• Colas, I.; Barakate, A.; Macaulay, M.; Schreiber, M.; Stephens, J.; Vivera, S.; Halpin, C.; Waugh, R.; Ramsay, L. (2019) Desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 (DMC1) in barley., Journal of Experimental Botany, 70, 2683-2698.
• Darrier, B.; Russel, J.; Milner, S.G.; Hedley, P.E.; Shaw, P.D.; Macaulay, M.; Ramsay, L.D.; Halpin, C.; Mascher, M.; Fleury, D.L.; Langridge, P.; Stein, N.; Waugh, R. (2019) A comparison of mainstream genotyping platforms for the evaluation and use of barley genetic resources., Frontiers in Plant Science, 10, Article No. 544.
• Looseley, M.E.; Griffe, L.L.; Büttner, B.; Wright, K.M.; Middlefell-Williams, J.; Bull, H.; Shaw, P.D.; Macaulay, M.; Booth, A.; Schweizer, G.; Russell, J.R.; Waugh, R.; Thomas, W.T.B.; Avrova, A. (2018) Resistance to Rhynchosporium commune in a collection of European spring barley germplasm., Theoretical and Applied Genetics, 131, 2513-2528.
• Bayer, M.; Ganal, M.; Hedley, P.E.; Macaulay, M.; Plieske, J.; Ramsay, L.; Rapazote-Flores, P.; Russell, J.; Shaw, P.; Thomas, W.; Waugh, R. (2017) Development and evaluation of a barley 50k iSelect SNP array., Frontiers in Plant Science, 8, Article No. 1792.
• Castro, A.J.; Cuesta-Marcos, A.; Hayes, P.M.; Locatelli, A.; Macaulay, M.; Mastandrea, N.; Silveira, M.; Thomas, W.T.B.; Viega, L. (2017) The completely additive effects of two barley phenology-related genes (eps2S and sdw1) are explained by specific effects at different periods within the crop growth cycle., Plant Breeding, 136, 663-670.
• Liller, C.B.; Walla, A.; Boer, M.P.; Hedley, P.; Macaulay, M.; Effgen, S.; von Korff, M.; van Esse, G.W.; Koornneef, M. (2017) Fine mapping of a major QTL for awn length in barley using a multiparent mapping population., Theoretical and Applied Genetics, 130, 269-281.
• Colas, I.; Macaulay, M.; Higgins, J.D.; Phillips, D.; Barakate, A.; Posch, M.; Armstrong, S.J.; Franklin, F.C.H.; Halpin, C.; Waugh, R.; Ramsay, L. (2016) A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10., New Phytologist, 212, 693-707.
• Travis, A.J.; Norton, G.J.; Datta, S.; Sarma, R.; Dasgupta, T.; Savio, F.L.; Macaulay, M.; Hedley, P.E.; McNally, K.L.; Sumon, M.H.; Islam, M.R.; Price, A.H. (2015) Assessing the genetic diversity of rice originating from Bangladesh, Assam and West Bengal., Rice, 8, Article No. 35.
• Phillips, D.; Jenkins, G.; Macaulay, M.; Nibau, C.; Wnetrzak, J.; Fallding, D.; Colas, I.; Oakey, H.; Waugh, R.; Ramsay, L. (2015) The effect of temperature on the male and female recombination landscape of barley., New Phytologist, 208, 421-429.
• Forsberg, N.E.G.; Russell, J.; Macaulay, M.; Leino, M.W.; Hagenblad, J. (2015) Farmers without borders – genetic structuring in century old barley (Hordeum vulgare)., Heredity, 114, 195-206.
• Bertholdsson, N.O.; Holefors, A.; Macaulay, M.; Crespo-Herrera, L.A. (2015) QTL for chlorophyll fluorescence of barley plants grown at low oxygen concentration in hydroponics to simulate waterlogging., Euphytica, 201, 357-365.
• Yelina, N.E.; Choi, K.; Chelysheva, L.; Macaulay, M.; de Snoo, B.; Wijnker, E.; Miller, N.; Drouaud, J.; Grelon, M.; Copenhaver, G.P.; Mezard, C.; Kelly, K.A.; Henderson, I.R. (2012) Epigenetic remodeling of meiotic crossover frequency in arabidopsis thaliana DNA methyltransferase mutants., PLoS Genetics, 8, Article No. e1002844.
• Houston, K.; Druka, A.; Bonar, N.; Macaulay, M.; Lundqvist, U.; Franckowiak, J.D.; Morgante, M.; Stein, N.; Waugh, R. (2012) Analysis of the barley bract suppression gene Trd1., Theoretical and Applied Genetics, 125, 33-45.
• Looseley, M.; Newton, A.C.; Atkins, S.D.; Fitt, B.D.L.; Fraaije, B.A.; Thomas, W.T.B.; Keith, R.; Macaulay, M.; Lynott, J.S.; Harrap, D. (2012) Genetic basis of control of Rhynchosporium secalis infection and symptom expression in barley., Euphytica, 184, 47-56.
• Hofinger, B.J.; Russell, J.R.; Bass, C.G.; Baldwin, T.; dos Reis, M.; Hedley, P.E.; Li, Y.; Macaulay, M.; Waugh, R.; Hammond-Kosack, K.E.; Kanyuka, K. (2011) An exceptionally high nucleotide and haplotype diversity and a signature of positive selection for the eIF4E resistance gene in barley are revealed by allele mining and phylogenetic analyses of natural populations., Molecular Ecology, 20, 3653-3668.
• Ramsay, L.; Comadran, J.; Druka, A.; Marshall, D.F.; Thomas, W.T.B.; Macaulay, M.; MacKenzie, K.; Simpson, C.G.; Fuller, J.; Bonar, N.; Hayes, P.M.; Lundqvist, U.; Franckowiak, J.D.; Close, T.J.; Muehlbauer, G.; Waugh, R. (2011) INTERMEDIUM-C, a modifier of lateral spikelet fertility in barley, is an ortholog of the maize domestication gene TEOSINTE BRANCHED 1., Nature Genetics, 43, 169-172.
• Cockram, J.; White, J.; Zuluaga, D.L.; Smith, D.; Comadran, J.; Macaulay, M.; Luo, Z.W.; Kearsey, M.J.; Werner, P.; Harrap, D.; Tapsell, C.; Liu, H.; Hedley, P.E.; Stein, N.; Schulte, D.; Steuernagel, B.; Marshall, D.F.; Thomas, W.T.B.; Ramsay, L.; Mackay, I.; Balding, D.J.; Waugh, R.; O’Sullivan, D.M.; Booer, C.; Pike, S.; Hamilton, G.; Jellis, G.; Davies, N.; Ross, A.; Bury, P.; Habgood, R.; Klose, S.; Vequaud, D.; Christerson, T.; Brosnan, J.; Newton, A.; Russel, J.; Shaw, P.; Bayles, R.; Wang, M. (2010) Genome-wide association mapping to candidate polymorphism resolution in the unsequenced barley genome, Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, pp. 21611-21616.
• Druka, A.; Franckowiak, J.D.; Lundqvist, U.; Bonar, N.; Alexander, J.; Guzy-Wrobelska, J.; Ramsay, L.; Druka, I.; Grant, I.; Macaulay, M.; Vendramin, V.; Shahinnia, F.; Radovic, S.; Houston, K.; Harrap, D.; Cardle, L.; Marshall, D.F.; Morgante, M.; Stein, N.; Waugh, R. (2010) Exploiting induced variation to dissect quantitative traits in barley., Biochemical Society Transactions, 38, 683-688.
• Rae, S.J.; Macaulay, M.; Ramsay, L.; Leigh, F.; Matthews, D.; O’Sullivan, D.M.; Donini, P.; Morris, P.C.; Powell, W.; Marshall, D.F.; Waugh, R.; Thomas, W.T.B. (2007) Molecular barley breeding., Euphytica, 158, 295-303.
• Ramsay, L.; Russell, J.R.; Macaulay, M.; Booth, A.; Thomas, W.T.B. (2004) Variation shown by molecular markers in barley: Genomic and genetic constraints., Aspects of Applied Biology, 72, 147-154.
• Macaulay, M.; Ramsay, L.; Powell, W.; Waugh, R. (2001) A representative, highly informative ‘genotyping set’ of barley SSRs., Theoretical and Applied Genetics, 102, 801-809.
• Cardle, L.; Ramsay, L.; Milbourne, D.; Macaulay, M.; Marshall, D.F.; Waugh, R. (2000) Computational and experimental characterization of physically clustered simple sequence repeats in plants., Genetics, 156, 847-854.

Books / chapters

• Arrieta, M.; Colas, I.; Macaulay, M.; Waugh, R.; Ramsay, L. (2020) A modular tray growth system for barley, In: Pradillo M. & Heckmann S. (eds.) Plant Meiosis. Methods in Molecular Biology, Humana, New York, Volume 2061, 367-379
• Ellis, R.P.; Macaulay, M.; Hackett, C.A.; Forster, B.P. (1998) The genetic control of ear emergence time in barley, Hordeum vulgare L.: Linkage between Vrn1, Bmy1, Wsp3 and a quantitative trait for time of ear emergence on chromosome 4H., In: Naqvi, S.S.M. (ed.). New Genetical Approaches to Crop Improvement. Atomic Energy Research Centre, Tando Jam 70060, Pakistan, pp619-630.
• Thomas, W.T.B.; Russell, J.R.; Bonar, N.; Booth, A.; Fuller, J.D.; Lawrence, P.; Macaulay, M.; Ramsay, L.; Waugh, R.; Powell, W. (1997) Simple sequence repeats SSRs and QTL mapping in barley crosses.., In: H. Buerstmayer & P. Ruckenbauer (eds.). Application of Marker Aided Selection in Cereal Breeding Programs. Eucarpia Section Cereals Meeting. Tulln, Austria, 28-29.

Technical / contract reports

• Macaulay, M.; Russell, J.R.; Ramsay, L.; Marshall, D.F.; Powell, W.; Waugh, R.; Thomas, W.T.B. (2004) Lessons from barley recommended lists., Annual Report of the Scottish Crop Research Institute for 2003/2004, pp144-145.
• Ramsay, L.; Caldwell, K.; Russell, J.R.; Macaulay, M.; Thomas, W.T.B.; Powell, W.; Waugh, R. (2003) Linkage disequilibrium in barley., Annual Report of the Scottish Crop Research Institute for 2002/2003, p114.
• Cardle, L.; Macaulay, M.; Marshall, D.F.; Milbourne, D.; Ramsay, L.; Waugh, R. (2000) SSR frequency and occurrence in plant genomes., Annual Report of the Scottish Crop Research Institute for 1999/2000, pp108-110.
• Thomas, W.T.B.; Newton, A.C.; Wilson, A.; Booth, A.; Macaulay, M.; Keith, R. (2000) Development of recombinant chromosome substitution lines – a barley resource., Annual Report of the Scottish Crop Research Institute for 1999/2000, pp99-100.
• Macaulay, M.; Provan, J.; Russell, J.R.; Fuller, J.D.; Dewar, H.; Milbourne, D.; Meyer, R.; Collins, A.; White, G.; Powell, W.; Waugh, R. (1996) Isolation, characterisation and use of SSRs as genetic markers., Annual Report of the Scottish Crop Research Institute for 1995, pp54-55.

Conference papers

• Looseley, M.E.; Newton, A.C.; Atkins, S.D.; Fitt, B.D.L.; Fraaije, B.A.; Thomas, W.T.B.; Keith, R.; Macaulay, M.; Lynott J.; Harrap, D.; Thirugnanasambandam, A. (2012) Genetic control of infection and symptom expression by Rhynchosporium commune in barley., Proceedings of Crop Protection in Northern Britain 2012, The West Park Conference Centre, Dundee, 28-29 February 2012.
• Moragues, M.; Russell, J.R.; Ceccarelli, S.; Grando, S.; Baum, M.; Macaulay, M.; Booth, A.; Waugh, R.; Ramsay, L.; Comadran, J.; Close, T.J.; de Young, J.; Flavell, A.J. (2008) High throughput SNP-based marker analysis of landrace barley., 10th International Barley Genetics Symposium, Alexandria, Egypt, 5-10 April 2008.
• Macaulay, M.; Ramsay, L.; Russell, J.R.; Marshall, D.F.; Waugh, R. (2004) Molecular markers to analyse breeding progress in barley., Aspects of Applied Biology, 72, 139-146.
• Macaulay, M.; Ramsay, L.; Waugh, R.; Thomas, W.T.B. (2004) Genes for success – what can we learn from recommended list trials?., Barley Genetics IX, Proceedings of 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June 2004, pp1040-1046.
• Russell, J.R.; Ramsay, L.; Booth, A.; Macaulay, M.; Cardle, L.; Marshall, D.F.; Powell, W.; Waugh, R. (2004) Development of gene based molecular markers in barley., Barley Genetics IX, Proceedings of 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June 2004.
• Ramsay, L.; Macaulay, M.; Thomas, W.T.B.; Druka, A.; Marshall, D.F.; Waugh, R. (2004) The semi-dwarfing gene sdw1 in European spring barley., Barley Genetics IX, Proceedings of 9th International Barley Genetics Symposium, Brno, Czech Republic, 20-26 June 2004, 321-325.
• Ellis, R.P.; Ramsay, L.; McLean, K.; Macaulay, M.; Waugh, R.; Powell, W. (2000) A molecular dissection of the genetic effects of domestication in barley., Proceedings of the 8th International Barley Genetics Symposium, Barley Genetics VIII (III), Adelaide, 22-27 October 2000, pp58-60.
• Waugh, R.; Ramsay, L.; Macaulay, M.; McLean, K.; Thomas, W.T.B.; Ellis, R.P.; Swanston, J.S.; Russell, J.R.; Cardle, L.; Harrower, B.E.; Hedley, P.E.; Powell, W.; Machray, G.C. (1999) Identification and improvement of components of barley malting quality., Proceedings of the 9th Australian Barley Technical Symposium, Melbourne, 12-16 September 1999, pp98-112.
• Macaulay, M.; Stephenson, P.; Gale, M.; Waugh, R. (1998) Development and assessment of SSR-based markers for application in genetical studies in barley and wheat., BBSRC PAGA/GAIT Workshop, Edinburgh, April 1998.
• Russell, J.R.; Fuller, J.D.; Macaulay, M.; Hatz, B.G.; Jahoor, A.; Powell, W.; Waugh, R. (1996) Direct comparisons of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs., VII International Barley Genetics Symposium, Saskatoon, Canada, 5 August 1996, pp295-297.
• Powell, W.; Baird, E.; Booth, A.; Lawrence, P.; Macaulay, M.; Bonar, N.; Young, G.; Thomas, W.T.B.; McNicol, J.W.; Waugh, R. (1996) Single locus and multi-locus molecular assays for barley breeding and research., VII International Barley Genetics Symposium, Saskatoon, Canada, 5 August 1996, pp174-181.

Conference posters / abstracts

• Shaw, P.; Raubach, S.; Macaulay, M.; Schreiber, M.; Milne, I.; Bayer, M.; Kilian, B. (2023) Going for BOLD Supporting the Biodiversity for Opportunities, Livelihoods and Development Project with Germinate and GridScore, Plant and Animal Genome 30, 13-18 January 2023. San Diego, USA & Online.
• Colas, I.; Macaulay, M.; Mittmann, S.; Ramsay, L.; Waugh, R. (2019) Using barley mutants to understand meiosis and recombination in cereals., COST INDEPTH SEB Conference, Spain, 8-12 December 2019.
• Ramsay, L.; Colas, I.; Macaulay, M.; Sandhu, A.; Armstrong, S.; Waugh, R. (2019) Desynaptic8 is a spontaneous mutation in X-Ray Repair Cross Complementing 2 (XRCC2) in barley. , British Meiosis Meeting, Aberystwyth, UK, 17-18 April 2019.
• Schreiber, M.; Barakate, A.; Uzrek, N.; Colas, I.; Lewandowska, D.; Macaulay, M.; Mittmann, S.; Arrieta, M.; Clavijo, B.; Wright, J.; Ramsay, L.; Waugh, R. (2018) Developing bioinformatics resources to study meiosis in the barley cultivar Golden Promise., Plant and Animal Genome, Sa Diego, USA, 13-17 January 2018.
• Barakate, A.; Schreiber, M.; Stephens, J.; Davidson, D.; Uzrek, N.; Macaulay, M.; Colas, I.; Lewandowska, D.; Sourdille, A.; Arrieta, M.; Mittmann, S.; Ramsay, L.; Waugh, R. (2018) Targeted mutagenesis of meiotic genes in barley (Hordeum vulgare): Understanding and manipulating meiotic crossovers., Monogram 2018, Norwich, UK, 24-26 April 2018.
• Colas, I.; Mittmann, S.;Macaulay, M.; Ramsay, L.; Waugh, R. (2018) Using barley mutants to understand meiosis and recombination in cereals., British Meiosis Meeting, Brighton, UK, 24-25 April 2018. (Poster)
• Barakate, A.; Stephens, J.; Colas, I.; Schreiber, M.; Lewandowska, D.; Uzrek, N.; Macaulay, M.; Arrieta, M.; Mittmann, S.; Ramsay, L.; Waugh, R. (2017) Understanding and manipulating meiotic crossovers in barley (Hordeum vulgare)., British Meiosis Meeting, Dundee, 19-20 April 2017. Poster
• Mittmann, S.; Ramsay, L.; Macaulay, M.; Waugh, R.; Colas, I. (2017) Discovery of novel E3 ubiquitin ligase by fine mapping and characterization of the barley desynaptic mutant des12., British Meiosis Meeting, Dundee, 19-20 April 2017. Poster.
• Mittmann, S.; Ramsay, L.; Macaulay, M.; Waugh, R.; Colas, I. (2016) Control of meiosis and recombination in barley; Characterization and fine mapping of the barley desynaptic mutant des12., EPSO/FESPB Meeting , Prague, 26-30 June 2016. Poster.
• Macaulay, M.; Colas, I.; Waugh, R.; Ramsay, L. (2014) Mapping and characterization of the barley desynaptic mutants., Plant and Animal Genome XXII, San Diego, California, USA, 12-16 January 2014.
• Colas, I.; Macaulay, M.; Mittmann, S.; Waugh, R.; Ramsay, L. (2014) Cytological characterization of the barley desynaptic mutants., Plant and Animal Genome XXII, San Diego, California, USA, 12-16 January 2014.
• Colas, I.; Macaulay, M.; Waugh, R.; Ramsay, L. (2013) Fine mapping and characterization of the barley desynpatic des10., EMBO Meiosis Conference, Germany, September 2013.
• Bull, H.; Keith, R.; Macaulay, M.; Hedley, P.E.; Thomas, W.T.B. (2012) The barley multi-cross mapping matrix., Crop Genomics and Crop Improvement, University of East Anglia, Norwich, 5-6 December 2012. (Abstract)
• Looseley, M.E.; Newton, A.C.; Atkins, S.D.; Fitt, B.D.L.; Fraaije, B.A.; Thomas, W.T.B.; Keith, R.; Macaulay, M.; Lynott, J.; Harrap, D. (2011) Resistance to Rhynchosporium secalis in a cross between winter and spring barley., Fourth International Workshop on Barley Leaf Blights, West Park Centre, Dundee, 27-29 June 2011.
• Comadran, J.; Ramsay, L.; William, T.; Macaulay, M.; Marshall, D.F.; Shaw, P.; Cockram, J.; White, J.; Mackay, I.; Liu, Z.; Kearsey, M.J.; O’Sullivan, D.; Waugh, R. (2011) Genetic selection footprints in elite barley cultivars., Plant and Animal Genome XIX, San Diego, California, USA, 15-19 January 2011. (Poster)
• Macaulay, M.; Ramsay, L.; Waugh, R.; Thomas, W.T.B. (2005) Prospects for marker assisted selection for nitrogen accumulation characters., ECO-PB/SUSVAR Abstracts, Dtiebergen, The Netherlands, 17-19 January 2005.
• Ramsay, L.; Macaulay, M.; Thomas, W.T.B.; Druka, A.; Marshall, D.F.; Waugh, R. (2004) The semi-dwarfing gene sdw1 in European spring barley., Plant & Animal Genome XII, San Diego, 10-14 January 2004, 187.
• Macaulay, M.; Stevenson, P.; Gale, M.; Waugh, R. (1997) Development and evaluation of SSRs in wheat and barley., BBSRC PAGA Annual Conference, Warwick, April 1997.
• Macaulay, M.; Stevenson, P.; Gale, M.; Waugh, R. (1996) Isolation and characterisation of simple sequence repeats from wheat and barley., BBSRC PAGA Annual Conference. Warwick, May 1996 (Poster).