Robert Hancock

Plant Biochemist
Cell and Molecular Sciences
T: +44(0)1382 568779

Robert Hancock is a senior biochemist and plant physiologist with 25 years post-qualification experience. His primary interests are concerned with the biochemical determinants and impact of biotic and abiotic stress on crop quality and how crop management and management systems can influence yield and quality. He works in soft fruits, potatoes and cereals where he has extensive industry links across the production and retail chain. His laboratory uses a range of physiological, biochemical, analytical and molecular techniques to understand the impact of stress on crop quality at a systems level. This research is supported by links with bioinformaticians working within Biomathematics and Statistics Scotland (BioSS), co-located at the James Hutton Institute.  Fundamental work is complemented by more applied aspects of research which are facilitated through close interactions with colleagues working in the area of molecular breeding as well as extensive interactions and joint research projects with industrial partners.

Research

My current research interests focus on the impact of plant genotype, the biotic and the abiotic environment on crop quality with particular focus on the role of reactive oxygen signalling and the cellular redox environment in modulating plant responses to environmental cues. I conduct a mixture of both fundamental and applied research adopting a physiological, biochemical and increasingly molecular approach. Primary crops of interest are potatoes and soft fruit.

The laboratory currently hosts three PhD students. Two of these research projects aim to understand the interaction between genetics and environment in the accumulation of phytochemicals associated with quality in soft fruit (blackcurrants and blueberries) and a further project is examining plant responses to aphids.

In blueberry we aim to understand the relationship between fruit chemistry and sensory attributes in collaboration with colleagues at the University of Strathclyde. In addition, we aim to understand the interplay between crop genetics and growing environment with respect to the accumulation of compounds providing potential health benefits to consumers (vitamin C, polyphenolic antioxidants). Our analytical and biochemical work feeds into a genetic map being produced by colleagues to allow the rapid breeding of blueberry cultivars suitable for production of fruit meeting UK consumer expectations.

A similar approach is being applied to understanding the accumulation of a range of phytochemicals in blackcurrant fruit. Here we aim to characterise genes associated with the accumulation of specific phytochemicals by developing a blackcurrant microarray to allow analysis of global gene expression throughout the course of fruit ripening. Fruit phytochemistry changes dramatically over the course of ripening and by combining phytochemical analysis with gene expression studies we aim to identify genes contributing to phytochemical accumulation. This will feed into the Hutton blackcurrant breeding programme providing candidate genes for cultivar improvement.

Microarray technologies, in combination with metabolome analysis has allowed us to characterise the response of the model plant Arabidopsis to aphid infestation. In collaboration with colleagues at the University of Leeds, we have demonstrated the divergence of local and systemic responses and identified cellular redox processes as key determinants of the plant response. This has been further verified by analysis of mutants that have low vitamin C levels and hence a more oxidised cellular redox environment. Furthermore, we have been able to demonstrate that redox processes are also key in the response of crops (potato) to aphid infestation.

The laboratory is also currently funded by the Scottish Government to develop an understanding of potato to heat stress in order to breed heat resistant cultivars. Our focus is on understanding the impact of high temperatures on carbon partitioning within the plant and the role of redox processes on carbon assimilation, transport and metabolism.

  • Innovate UK Strawberry molecular breeding 10015546 2022-2025 (£298,987) (PI)
  • BBSRC GCRF ZIRON Pulse BB/T008865/1 2020 – 2023 (£1M) (Co-I)

Past research

My initial work at the Institute took a biochemical and physiological approach to understanding the accumulation of vitamin C in blackcurrant fruit and potato tubers. We were able to demonstrate that in addition to biosynthesis within the fruit or tuber, vitamin C was transported long distance from leaves via the phloem. Additional work involved the development of engineered yeast strains capable of vitamin C biosynthesis. I have also worked on key plant developmental programmes including bud dormancy, potato dormancy and potato tuberisation. Applied work included a Scottish Enterprise funded programme developing methods for the extension of shelf-life in minimally processed (fresh-cut) fruit and vegetables.

Publications

The following Publications have not yet been migrated to the James Hutton Institute's Pure service and relate to the research outputs from the two legacy organisations: The Macaulay Land Use Research Institute and The Scottish Crop Research Institute.

Journals

Books / chapters

  • Hancock, R.D.; Stewart, D. (2010) Enhancing the nutritional quality of fruit juices: advanced technologies for juice extraction and pasteurization., In: Bagchi, D., Lau, F.C. & Ghosh, D. (eds.). Biotechnology In Functional Foods and Nutraceuticals. CRC Press, Boca Raton, FLA, USA, 463-482.

Technical / contract reports

  • Kerchev, P.I.; Saraswat, V.; Hedley, P.E.; Morris, J.; Fenton, B.; Foyer, C.H.; Hancock, R.D. (2011) Understanding local and systemic responses to aphid infestation as a tool towards breeding aphid resistant crops., Annual Report of the Scottish Crop Research Institute for 2010, pp40-42.
  • Ross, H.A.; Morris, W.L.; Ducreux, L.J.M.; Hancock, R.D.; Verrall, S.R.; Morris, J.A.; Stewart, D.; Hedley, P.E.; McDougall, G.J.; Taylor, M.A. (2011) The role of pectin methyl esterase in determining potato tuber textural quality., Annual Report of the Scottish Crop Research Institute for 2010, pp32-33.
  • Walker, P.G.; Pont, S.D.A.; Marquis, N.; Hancock, R.D. (2008) Mechanism of vitamin C accumulation in blackcurrant fruit., Annual Report of the Scottish Crop Research Institute for 2007, pp34-35.
  • Mazzitelli, L.; Hancock, R.D.; Haupt, S.; McNicol, J.W.; Viola, R.; Brennan, R.M.; Hedley, P.E.; Taylor, M.A. (2006) Understanding dormancy release in raspberry buds., Annual Report of the Scottish Crop Research Institute for 2006, pp32-33.
  • Pont, S.D.A.; Walker, P.G.; Lamond, S.; Fenton, B.; Hancock, R.D. (2005) Aphid reproduction is modulated by phloem ascorbic acid – novel targets for pest control?., Annual Report of the Scottish Crop Research Institute for 2004/2005, pp34-35.
  • Hancock, R.D.; McRae, D.; Haupt, S.; Viola, R. (2003) Biosynthesis of vitamin C in plant phloem., Annual Report of the Scottish Crop Research Institute for 2002/2003, pp110-111.
  • Hancock, R.D.; Viola, R. (2002) Ascorbic acid biosynthesis in higher plants and micro-organisms., Annual Report of the Scottish Crop Research Institute for 2001/2002, pp135-139.

Conference papers

  • Iannetta, P.P.M.; Escobar, N.M.; Ross, H.A.; Souleyre, E.; Hancock, R.D.; Fairlie, J.; Davies, H.V. (2000) Tricarboxylic enzymes in ripening strawberry fruits., Proceedings of the Fourth International Strawberry Symposium, Tampere, Finland, 9-14 July 2000. ISHS Acta Horticulturae (2002), 567, 731-733.

Conference posters / abstracts

  • Jarret, D.; Hancock, R.D.; Hedley, P.E.; Flavell, A.J.; Brennan, R.M. (2011) Towards an understanding of genetic control of processing quality traits in blackcurrant., Xth International Rubus and Ribes Symposiumn Zlatibor, Serbia, 22-26 June 2011. (Poster)
  • Kerchev, P.I.; Fenton, B.; Foyer, C.H.; Hancock, R.D. (2011) A role for redox components in the local and systmeic respones of Arabidopsis thaliana to aphid infestation., 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, Budapest, Hungary, 5-8 July 2011 (Poster).
  • Pont, S.D.A.; Ponte, A.; Shepherd, L.V.T.; Davies, H.V.; Hancock, R.D. (2011) Towards reduced acrylamide potato products: Analysis of precuros accumulation pathways using 13C-labelling in conjunction with GC/MS., PlantEngine Plant Metabolic Engineering for High Value Products, Murcia, Spain,17-18 November 2011.
  • McCallum, S.; Graham, J.; Hackett, C.A.; Hancock, R.D.; McKenzie, B.M.; Hallett, P.D.; Jorgensen, L.; Gordon, S.; Woodhead, M.; Brennan, R.M.; Rowland, L.J.; Bassil, N.; Olmstead, J.; Buck, E.; Hancock, J. (2010) Investigating the suitability of blueberry germplasm for UK production and developing tools for long-term breeding., Proceedings of the North American Blueberry Research and Extension Workers Conference (NABREW), Kalamazoo, Michigan, USA, 25-28 July 2010. (Poster)
  • Birch, A.N.E.; Shepherd, T.; Hancock, R.D.; Goszcz, K. (2010) Sugar sensing in inducible plant defence against multiple pest types., Scottish Plant Biology Meeting, University of Dundee, 22 April 2010.
  • Kerchev, P.; Foyer, C.H.; Fenton, B.; Hancock, R.D. (2009) Investigating roles for reactive oxygen in plant-aphid interactions., Plant ROS 2009: Society for Free Radical Research International Plant Oxygen Group Meeting on Reactive Oxygen and Nitrogen Species. Helsinki, Finland, 8-10 July 2009. (Poster).
  • Birch, A.N.E.; Shepherd, T.; Hancock, R.D.; Goszcz, K. (2009) Understanding sugar sensing in induced plant defences and stress tolerance., International Society of Chemical Ecology, Neuchatel, Switzerland, 23-25 August 2009.
  • Kerchev, P.; Foyer, C.H.; Fenton, B.; Hancock, R.D. (2009) Reactive oxygen and antioxidants modulate the interaction between Myzus persicae (Sulzer) and plant hosts., 8th International Symposium on Aphids, Catania, Italy, 8-12 June 2009 (Poster).
  • Mazzitelli, L.; Pont, S.D.A.; Walker, P.G.; Haupt, S.; Tilsner, J.; Taylor, M.A.; Hedley, P.E.; McNicol, J.W.; Hancock, R.D.; Viola, R. (2006) Molecular characterisation of dormancy phase transition in raspberry (Rubus idaeus L.) buds., Scottish Plant Molecular Biology Group Meeting. St Andrews, 13 January 2006 (Poster).
  • Mazzitelli, L.; Hein, I.; Hedley, P.E.; Haupt, S.; Tilsner, J.; Williamson, S.; Morris, J.; Pont, S.D.A.; Hancock, R.D.; Brennan, R.M.; Viola, R.; McNicol, J.W.; Russell, J.R.; Taylor, M.A. (2006) Microarray-based gene expression studies of dormancy phase transition in raspberry (Rubus idaeus L.) buds., 3rd International Rosaceae Conference. Napier, New Zealand, 19-22 March 2006 (Poster).
  • Hancock, R.D. (2006) Thermal treatments for enhancing shelf life of minimally processed foods., Excellence in Food Manufacture 2006, London, 1 November 2006 (Poster).
  • Hancock, R.D.; Walker, P.G.; Pont, S.D.A.; Smirnoff, N.; Viola, R. (2006) Ascorbic acid conjugates in phloem exudates of Cucurbitaceae., Proceedings of the 15th Congress of the Federation of European Societies of Plant Biology, Lyon, France, 17-21 July 2006 (Poster).
  • Walker, P.G.; Pont, S.D.A.; Viola, R.; Hancock, R.D. (2006) L-ascorbic acid accumulation in fruit of blackcurrant (Ribes nigrum L.)., Proceedings of the 15th Congress of the Federation of European Societies of Plant Biology, Lyon, France, 17-21 July 2006 (Poster).
  • Mazzitelli, L.; Pont, S.D.A.; Walker, P.G.; Haupt, S.; Tilsner, J.; Taylor, M.A.; Hedley, P.E.; McNicol, J.W.; Hancock, R.D.; Viola, R. (2005) Molecular characterisation of dormancy phase transition in raspberry (Rubus idaeus L.) buds., SEB Annual Main Meeting, Barcelona, 11-15 July 2005 (Poster).
  • Frusciante, L.; Di Matteo, A.; Hancock, R.D.; Ross, H.A.; Ercolano, M.R.; Viola, R. (2005) Manipulation of L-ascorbic acid biosynthesis in potato via reverse genetic approaches., 16th Triennial Conference of the European Association for Potato Research, Bilbao, Spain, 17-22 July 2005 (Poster).
  • Hancock, R.D.; Pont, S.D.A.; Fenton, B. (2005) Ascrobic acid modulates aphid success on potato: a role for reactive oxygen species?., Oxygen Metabolism, ROS and Redox Signalling in Plants, Bristol, 18-20 December 2005 (Poster).
  • Walker, P.G.; Viola, R.; Pont, S.D.A.; Brennan, R.M.; Hancock, R.D. (2005) Genotypic influence on L-ascorbic acid accumulation in blackcurrant (Ribes nigrum) fruit., Proceedings of the SEB Annual Conference, Barcelona, 11-15 July 2005 (Poster).
  • Di Matteo, A.; Hancock, R.D.; Ross, H.A.; Frusciante, L.; Viola, R. (2004) Characterisation of Chlorella pyrenoidosal accumulating mutants: identification of an enhanced biosynthetic enzyme activity and cloning of the putative gene from Arabidopsis thaliana into tobacco cells., Proceedings of the XLVIII Italian Society of Agricultural Genetics, Lecce, Italy, 15-18 September 2004 (Poster).
  • Di Matteo, A.; Hancock, R.D.; Ross, H.A.; Frusciante, L.; Viola, R. (2003) Characterisation of Chlorella pyrenoidosa L-ascorbic acid accumulating mutants: Identification of an enhanced biosynthetic enzyme activity and cloning of the putative gene from Arabidopsis thaliana., Proceedings of SEB 2003, Southampton, 134. (Poster).
  • Hancock, R.D.; Viola, R. (2001) Potential of engineering the plant L-ascorbic acid (vitamin C) biosynthetic pathway into the yeast Saccharomyces cerevisiae., Scottish Plant Cell and Molecular Biology Group Meeting, Dundee, 26 January 2001 (Poster).

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