Ingo Hein
Current research interests
My research aims to identify and clone more durable resistances towards major potato pathogen.
All microbes trigger immune responses in plants via host receptor-mediated recognition of PAMPs. Adapted pathogens suppress or otherwise manipulate PAMP-triggered immunity (PTI). They do this by secretion of virulence factors, called effectors, which re-programme host metabolism to the benefit of the pathogen.
Effector-triggered suppression of PTI is the pathogen’s front-line of attack in overcoming plant defences. When a successful pathogen has suppressed PTI, plants possess a second layer of inducible defence in the form of resistance (R) genes, the products of which typically encode NB-LRR proteins that detect effectors (termed avirulence proteins; AVRs).
In a “paradigm-shift” from conventional disease resistance breeding we exploit our knowledge of recent dramatic changes in the pathogen population to unravel the underlying variation in pathogen effectors, using this to drive the search for durable resistance.
A central component of our research is the exploitation of the recently sequenced potato genome and use of second generation sequencing and novel DNA capture technology to greatly accelerate discovery and isolation of functional resistance genes.
Current projects include:
- Identification and cloning of new genetic variations in Solanum species for resistance to Phytophthora infestans.
- Identification of the Phytophthora infestans effectors underpinning Ph2 and Ph3 resistance in tomato.Identification of potato NB-LRR genes and development of a second generation sequencing approach to clone functional R genes more rapidly
- Phytophthora infestans effector Avr3a diversity and function.
- Enhancing the recognitions specificity of known Solanum resistance genes in vitro.
Past research
- 2010 – present: Principal Investigator potato disease resistance group.
-
2006 – 2010: Senior Postdoctoral Researcher (Band 5 since 2008; SCRI)
Studying potato disease resistance to Phytophthora infestans. -
2003 – 2006: Postdoctoral Researcher (Band 6; SCRI)
Elucidating disease resistance pathways in potato and barley. Generation of genomic tools for studying root rot resistance in raspberry. -
March 2003: Doctor of Philosophy
Thesis title: Discovering Resistance Pathways in Response to Elicitors and Powdery Mildew Attack in Barley; University of Dundee, Department of Life Science, Dundee, United Kingdom -
October 1999: Diplom Biologe (Master in Biology)
Thesis title: Functional characterisation of a Homeobox family in Flaveria
Heinrich-Heine-University Düsseldorf, Institut für Entwicklungs- & Molekularbiologie der Pflanzen, Düsseldorf, Germany
Bibliography
- Thilliez, G.J.A.; Armstrong, M.R.; Lim, T.Y.; Baker, K.; Jouet, A.; Ward, B.; van Oosterhout, C.; Jones, J.D.G.; Huitema, E.; Birch, P.R.J.; Hein, I. (2019) Pathogen enrichment sequencing (PenSeq) enables population genomic studies in oomycetes., New Phytologist, 221, 1634-1648.
- Armstrong, M.R.; Vossen, J.; Lim, T.Y.; Hutten, R.C.B.; Xu, J.F.; Strachan, S.M.; Harrower, B.; Champouret, N.; Gilroy, E.M.; Hein I. (2019) Tracking disease resistance deployment in potato breeding by enrichment sequencing., Plant Biotechnology Journal, 17, 540-549.
- Wang, S.; McLellan, H.; Bukharova, T.; He, Q.; Murphy, F.; Shi, J.; Sun, S.; van Weymers, P.; Ren, Y.; Thilliez, G.; Wang, H.; Chen, X.; Engelhardt, S.; Vleeshouwers, V.; Gilroy, E.M.; Whisson, S.W.; Hein, I.; Wang, X.; Tian, Z.; Birch, P.R.J.; Boevink, P.C. (2019) Phytophthora infestans RXLR effectors act in concert at diverse subcellular localisations to enhance host colonisation., Journal of Experimental Botany, 70, 343-356.
- Jouet, A.; Saunders, D.G.O.; McMullan, M.; Ward, B.; Furzer, O.; Jupe, F.; Cevik, V.; Hein, I.; Thilliez, G.J.A.; Holub, E.; van Oosterhout, C.; Jones, J.D.G. (2018) Albugo candida race diversity, ploidy and host-associated microbes revealed using DNA sequence capture on diseased plants in the field., New Phytologist. Published online.
- Jiang, R.; Li, J.C.; Tian, Z.D.; Du, J.; Armstrong, M.; Baker, K.; Lim, J.T.Y.; Vossen, J.H.; He, H.; Portal, L.; Zhou, J.; Bonierbale, M.; Hein, I.; Lindqvist-Kreuze, H.; Xie, C.H. (2018) Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8., Journal of Experimental Botany, 69, 1545-1555.
- He, Q.; Naqvi, S.; McLellan, H.; Boevink, P.C.; Champouret, N.; Hein, I.; Birch, P.R.J. (2018) Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70., Proceedings of the National Academy of Sciences of the USA, 115, E7834-E7843.
- Chen, X.; Lewandowska, D.; Armstrong, M.R.; Baker, K.; Lim, T.Y.; Bayer, M.; Harrower, B.; McLean, K.; Jupe, F.; Witek, K.; Lees, A.K.; Jones, J.D.; Bryan, G.J.; Hein, I. (2018) Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies., Theoretical and Applied Genetics, 131, 1287-1297.
- Baker, K.; Stephen, G.; Strachan, S.; Armstrong, M.; Hein, I. (2018) BLASTmap: A Shiny-based application to visualize BLAST results as interactive heat maps and a tool to design gene-specific baits for bespoke target enrichment sequencing., In: Ma, W. & Wolpert, T. (eds.). Plant Pathogenic Fungi and Oomyctes. Methods in Molecular Biology, Humana Press, New York, Volume 1848, pp199-206.
- Paajanen, P.; Kettleborough, G.; Lopez-Girona, E.; Giolai, M.; Heavens, D.; Baker, D.; Lister, A.; Wilde, G.; Hein, I.; Macaulay, I.; Bryan, G.J.; Clark, M.D. (2017) A critical comparison of technologies for a plant genome sequencing project., BioRxiv, 11 October 2017.
- Giolai, M.; Paajanen, P.; Verweij, W.; Percival-Alwyn, L.; Baker, D.; Witek, K.; Jupe, F.; Bryan, G.; Hein, I.; Jones, J.D.G.; Clark, M.D. (2016) Targeted capture and sequencing of gene-sized DNA molecules., Biotechniques, 61, 315-322.
- Van Weymers, P.S.M.; Baker, K.; Chen, X.W.; Harrower, B.; Cooke, D.E.L.; Gilroy, E.M.; Birch, P.R.J.; Thilliez, G.J.A.; Lees, A.K.; Lynott, J.S.; Armstrong, M.R.; McKenzie, G.; Bryan, G.J.; Hein, I. (2016) Utilizing 'Omic' technologies to identify and prioritize novel sources of resistance to the oomycete pathogen Phytophthora infestans in potato germplasm collections., Frontiers in Plant Science, 7, Article No. 762.
- Boevink, P.C.; Wang, X.D.; McLellan, H.; He, Q.; Naqvi, S.; Armstrong, M.R.; Zhang, W.; Hein, I.; Gilroy, E.M.; Tian, Z.D.; Birch, P.R.J. (2016) A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease., Nature Communications, 7, Article No. 10311.
- Destefanis, M.; Nagy, I.; Rigney, B.; Bryan, G.J.; McLean, K.; Hein, I.; Griffin, D.; Milbourne, D. (2015) A disease resistance locus on potato and tomato chromosome 4 exhibits a conserved multipartite structure displaying different rates of evolution in different lineages., BMC Plant Biology, 15, Article No. 255.
- Wiesel, L.; Davis, J.L.; Milne, L.; Fernandez, V.R.; Herold, M.B.; Williams, J.M.; Morris, J.; Hedley, P.E.; Harrower, B.; Newton, A.C.; Birch, P.R.J.; Gilroy, E.M.; Hein, I. (2015) A transcriptional reference map of defence hormone responses in potato., Scientific Reports, 5, Article No. 15229.
- Feechan, A.; Turnbull, D.; Stevens, L.J.; Engelhardt, S.; Birch, P.R.J.; Hein, I.; Gilroy, E.M. (2015) The hypersensitive response in PAMP- and effector-triggered immune responses., In: Gunawardena, A. & McCabe, P. (eds.). Plant Programmed Cell Death. Springer, New York, Chapter 10, pp235-258.
- Wiesel, L.; Newton, A.C.; Elliot, I.; Booty, D.; Gilroy, E.M.; Birch, P.R.J.; Hein, I. (2014) Molecular effects of resistance elicitors from biological origin and their potential for crop protection., Frontiers in Plant Science, 5, Article No. 655.
- Tomcynska, I.; Jupe, F.; Hein, I.; Marczewski, W.; Sliwka, J. (2014) Hypersensitive response to Potato virus Y in potato cultivar Sarpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX., Molecular Breeding, 34, 471-480.
- Chapman, S.; Stevens, L.J.; Boevink, P.C.; Engelhardt, S.; Alexander, C.J.; Harrower, B.; Champouret, N.; McGeachy, K.; Van Weymers, P.S.M.; Chen, X.; Birch, P.R.J.; Hein, I. (2014) Detection of the virulent form of AVR3a from phytophthora infestans following artificial evolution of potato resistance gene R3a., PLoS ONE, 9, Article No. e110158.
- Jupe, F.; Chen, X.; Verweij, W.; Witek, K.; Jones, J.D.G.; Hein I. (2014) Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants., In: Birch, P.R.J., Jones, J.T. & Bos, J.I.B. (eds.). Plant-Pathogen Interactions: Methods in Molecular Biology. Volume 1127, Humana Press, New York, 2nd ed, Chapter 22, pp291-303.
- Jupe, F.; Witek, K.; Verweij, W.; Sliwka, J.; Pritchard, L.; Etherington, G.J.; Maclean, D.; Cock, P.J.; Leggett, R.M.; Bryan, G.J.; Cardle, L.; Hein, I.; Jones, J.D.G. (2013) Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations., Plant Journal, 76, 530-544.
- Engelhardt, S.; Boevink, P.C.; Armstrong, M.R.; Ramos, M.; Hein, I.; Birch, P.R.J. (2012) Relocalization of late blight resistance protein R3a to endosomal compartments is associated with effector recognition and required for the immune response., The Plant Cell, 24, 5142-5158.
- Saunders, D.G.O.; Breen, S.; Win, J.; Schornack, S.; Hein, I.; Bozkurt, T.O.; Champouret, N.; Vleeshouwers, G.A.A.; Birch, P.R.J.; Gilroy, E.M.; Kamoun, S. (2012) Host protein BSL1 associates with phytophthora infestans RXLR effector AVR2 and the solanum demissum immune receptor R2 to mediate disease resistance., The Plant Cell, 24, 3420-3434.
- Cooke, D.E.L.; Cano, L.M.; Raffaele, S.; Bain, R.A.; Cooke, L.R.; Etherington, G.J.; Deahl, K.L.; Farrer, R.A.; Gilroy, E.M.; Goss, E.M.; Grunwald, N.J.; Hein, I.; Maclean, D.; McNicol, J.W.; Randall, E.; Oliva, R.F.; Pel, M.A.; Shaw, D.S.; Squires, J.N.; Taylor, M.C.; Vleeshouwers, V.G.A.A.; Birch, P.R.J.; Lees, A.K.; Kamoun, S. (2012) Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen., PLoS Pathogens, 8, Article No. e1002940.
- Jupe, F.; Pritchard, L.; Etherington, G.; MacKenzie, K.; Cock, P.J.A.; Wright, F.; Sharma, S.K.; Bolser, D.; Bryan, G.J.; Jones, J.; Hein, I. (2012) Identification and localisation of the NB-LRR gene family within the potato genome., BMC Genomics,13, 1-14.
- Birch, P.R.J.; Bryan, G.J.; Fenton, B.; Gilroy, E.M.; Hein, I.; Jones, J.T.; Prashar, A.; Taylor, M.A.; Torrance, L.; Toth, I.K. (2012) Crops that feed the world 8: potato: are the trends of increased global production sustainable?, Food Security, 4, 477-508.
- Whisson, S.C.; Avrova, A.O.; Boevink, P.C.; Armstrong, M.R.; Seman, Z.A.; Hein, I.; Birch, P.R.J. (2011) Exploiting knowledge of pathogen effectors to enhance late blight resistance in potato., Potato Research, 54, 325-340.
- Sun, X.L.; Gilroy, E.M.; Chini, A.; Nurmberg, P.L.; Hein, I.; Lacomme, C.; Birch, P.R.J.; Hussain, A.; Yun, B.W.; Loake, G.J. (2011) ADS1 encodes a MATE-transporter that negatively regulates plant disease resistance., New Phytologist, 192, 471-482.
- Gilroy, E.M.; Breen, S.; Whisson, S.C.; Squires, J.; Hein, I.; Kaczmarek, M.; Turnbull, D.; Boevink, P.; Lokossou, A.; Cano, L.M.; Morales, J.; Avrova, A.O.; Pritchard, L.; Randall, E.; Lees, A.K.; Govers, F.; van West, P.; Kamoun, S.; Vleeshouwers, V.G.A.A.; Cooke, D.E.L.; Birch, P.R.J. (2011) Presence/absence, differential expression and sequence polmorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants., New Phytologist, 191, 763-776.
- Gilroy, E.M.; Taylor, R.M.; Hein, I.; Boevink, P.; Sadanandom, A.; Birch, P.R.J. (2011) CMPG1-dependent cell death follows perception of diverse pathogen elicitors at the host plasma membrane and is suppressed by Phytophthora infestans RXLR effector AVR3a., New Phytologist, 190, 653-666.
- Hein, I.; Birch, P.R.J.; Chapman, S.N.; Bryan, G.J. (2011) Pathogen effector driven search for more durable potato late blight resistance., Annual Report of the Scottish Crop Research Institute for 2010, pp38-40.
- Rietman, H.; Champouret, N.; Hein, I.; Niks, R.E.; Vleeshouwers, V.G.A.A. (2010) Plant and oomycetes, an intimate relationship: Co-evolutionary principles and impact on agricultural practice., CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 5, 1-17.
- Bos, J.I.B.; Armstrong, M.R.; Gilroy, E.M.; Boevink, P.; Hein, I.; Taylor, R.M.; Tian, Z.D.; Engelhardt, S.; Vetukuri, R.R.; Harrower, B.E.; Dixelius, C.; Bryan, G.J.; Sadanandom, A.; Whisson, S.C.; Kamoun, S.; Birch, P.R.J. (2010) Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 9909-9914.
- Avrova, A.O.; Birch, P.R.J.; Hein, I.; Jones, J.T.; Pritchard, L. (2010) Functional analysis of pathogen effectors: how to go from genomics to durable resistance., Annual Report of the Scottish Crop Research Institute for 2009, 39-41.
- Hein, I.; Birch, P.R.J.; Danan, S.; Lefebvre, V.; Odeny, D.A.; Gebhardt, C.; Trognitz, F.; Bryan, G.J. (2009) Progress in mapping and cloning qualitative and quantitative resistance against Phytophthora infestans in potato and its wild relatives., Potato Research, 52, 215-227.
- Hein, I.; Gilroy, E.M.; Armstrong, M.R.; Birch, P.R.J. (2009) The zig-zag-zig in oomycete-plant interactions., Molecular Plant Pathology, 10, 547-562.
- Crampton, B.G.; Hein, I.; Berger, D.K. (2009) Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)., Molecular Plant Pathology, 10, 291-304.
- Graham, J.; Hackett, C.A.; Smith, K.; Woodhead, M.; Hein, I.; McCallum, S. (2009) Mapping QTLs for developmental traits in raspberry from bud break to ripe fruit., Theoretical and Applied Genetics, 118, 1143-1155.
- Song, B.K.; Hein, I.; Druka, A.; Waugh, R.; Marshall, D.F.; Nadarajah, K.; Yap, S.J.; Ratnam, W. (2009) The 172-kb genomic DNA region of the O. rufipogon ylg1.1 locus: comparative sequence analysis with O. sativa ssp. Japonica and O. sativa ssp. Indica., Functional and Integrative Genomics, 9, 97-108.
- Birch, P.R.J.; Boevink, P.; Gilroy, E.M.; Hein, I.; Pritchard, L.; Whisson, S.C. (2008) Oomycete RXLR effectors: delivery, functional redundancy and durable disease resistance., Current Opinion in Plant Biology, 11, 373-379.
- Sharma, S.K.; Millam, S.; Bryan, G.J.; Hein, I. (2008) Cloning and molecular characterisation of a potato SERK gene transcriptionally induced during initiation of somatic embryogenesis., Planta, 228, 319-330.
- Thilliez, G.J.A.; Armstrong, M.R.; Lim, T.Y.; Baker, K.; Jouet, A.; Ward, B.; van Oosterhout, C.; Jones, J.D.G.; Huitema, E.; Birch, P.R.J.; Hein, I. (2019) Pathogen enrichment sequencing (PenSeq) enables population genomic studies in oomycetes., New Phytologist, 221, 1634-1648.
- Armstrong, M.R.; Vossen, J.; Lim, T.Y.; Hutten, R.C.B.; Xu, J.F.; Strachan, S.M.; Harrower, B.; Champouret, N.; Gilroy, E.M.; Hein I. (2019) Tracking disease resistance deployment in potato breeding by enrichment sequencing., Plant Biotechnology Journal, 17, 540-549.
- Wang, S.; McLellan, H.; Bukharova, T.; He, Q.; Murphy, F.; Shi, J.; Sun, S.; van Weymers, P.; Ren, Y.; Thilliez, G.; Wang, H.; Chen, X.; Engelhardt, S.; Vleeshouwers, V.; Gilroy, E.M.; Whisson, S.W.; Hein, I.; Wang, X.; Tian, Z.; Birch, P.R.J.; Boevink, P.C. (2019) Phytophthora infestans RXLR effectors act in concert at diverse subcellular localisations to enhance host colonisation., Journal of Experimental Botany, 70, 343-356.
- Jouet, A.; Saunders, D.G.O.; McMullan, M.; Ward, B.; Furzer, O.; Jupe, F.; Cevik, V.; Hein, I.; Thilliez, G.J.A.; Holub, E.; van Oosterhout, C.; Jones, J.D.G. (2018) Albugo candida race diversity, ploidy and host-associated microbes revealed using DNA sequence capture on diseased plants in the field., New Phytologist. Published online.
- Jiang, R.; Li, J.C.; Tian, Z.D.; Du, J.; Armstrong, M.; Baker, K.; Lim, J.T.Y.; Vossen, J.H.; He, H.; Portal, L.; Zhou, J.; Bonierbale, M.; Hein, I.; Lindqvist-Kreuze, H.; Xie, C.H. (2018) Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8., Journal of Experimental Botany, 69, 1545-1555.
- He, Q.; Naqvi, S.; McLellan, H.; Boevink, P.C.; Champouret, N.; Hein, I.; Birch, P.R.J. (2018) Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70., Proceedings of the National Academy of Sciences of the USA, 115, E7834-E7843.
- Chen, X.; Lewandowska, D.; Armstrong, M.R.; Baker, K.; Lim, T.Y.; Bayer, M.; Harrower, B.; McLean, K.; Jupe, F.; Witek, K.; Lees, A.K.; Jones, J.D.; Bryan, G.J.; Hein, I. (2018) Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies., Theoretical and Applied Genetics, 131, 1287-1297.
- Paajanen, P.; Kettleborough, G.; Lopez-Girona, E.; Giolai, M.; Heavens, D.; Baker, D.; Lister, A.; Wilde, G.; Hein, I.; Macaulay, I.; Bryan, G.J.; Clark, M.D. (2017) A critical comparison of technologies for a plant genome sequencing project., BioRxiv, 11 October 2017.
- Giolai, M.; Paajanen, P.; Verweij, W.; Percival-Alwyn, L.; Baker, D.; Witek, K.; Jupe, F.; Bryan, G.; Hein, I.; Jones, J.D.G.; Clark, M.D. (2016) Targeted capture and sequencing of gene-sized DNA molecules., Biotechniques, 61, 315-322.
- Van Weymers, P.S.M.; Baker, K.; Chen, X.W.; Harrower, B.; Cooke, D.E.L.; Gilroy, E.M.; Birch, P.R.J.; Thilliez, G.J.A.; Lees, A.K.; Lynott, J.S.; Armstrong, M.R.; McKenzie, G.; Bryan, G.J.; Hein, I. (2016) Utilizing 'Omic' technologies to identify and prioritize novel sources of resistance to the oomycete pathogen Phytophthora infestans in potato germplasm collections., Frontiers in Plant Science, 7, Article No. 762.
- Boevink, P.C.; Wang, X.D.; McLellan, H.; He, Q.; Naqvi, S.; Armstrong, M.R.; Zhang, W.; Hein, I.; Gilroy, E.M.; Tian, Z.D.; Birch, P.R.J. (2016) A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease., Nature Communications, 7, Article No. 10311.
- Destefanis, M.; Nagy, I.; Rigney, B.; Bryan, G.J.; McLean, K.; Hein, I.; Griffin, D.; Milbourne, D. (2015) A disease resistance locus on potato and tomato chromosome 4 exhibits a conserved multipartite structure displaying different rates of evolution in different lineages., BMC Plant Biology, 15, Article No. 255.
- Wiesel, L.; Davis, J.L.; Milne, L.; Fernandez, V.R.; Herold, M.B.; Williams, J.M.; Morris, J.; Hedley, P.E.; Harrower, B.; Newton, A.C.; Birch, P.R.J.; Gilroy, E.M.; Hein, I. (2015) A transcriptional reference map of defence hormone responses in potato., Scientific Reports, 5, Article No. 15229.
- Wiesel, L.; Newton, A.C.; Elliot, I.; Booty, D.; Gilroy, E.M.; Birch, P.R.J.; Hein, I. (2014) Molecular effects of resistance elicitors from biological origin and their potential for crop protection., Frontiers in Plant Science, 5, Article No. 655.
- Tomcynska, I.; Jupe, F.; Hein, I.; Marczewski, W.; Sliwka, J. (2014) Hypersensitive response to Potato virus Y in potato cultivar Sarpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX., Molecular Breeding, 34, 471-480.
- Chapman, S.; Stevens, L.J.; Boevink, P.C.; Engelhardt, S.; Alexander, C.J.; Harrower, B.; Champouret, N.; McGeachy, K.; Van Weymers, P.S.M.; Chen, X.; Birch, P.R.J.; Hein, I. (2014) Detection of the virulent form of AVR3a from phytophthora infestans following artificial evolution of potato resistance gene R3a., PLoS ONE, 9, Article No. e110158.
- Jupe, F.; Witek, K.; Verweij, W.; Sliwka, J.; Pritchard, L.; Etherington, G.J.; Maclean, D.; Cock, P.J.; Leggett, R.M.; Bryan, G.J.; Cardle, L.; Hein, I.; Jones, J.D.G. (2013) Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations., Plant Journal, 76, 530-544.
- Engelhardt, S.; Boevink, P.C.; Armstrong, M.R.; Ramos, M.; Hein, I.; Birch, P.R.J. (2012) Relocalization of late blight resistance protein R3a to endosomal compartments is associated with effector recognition and required for the immune response., The Plant Cell, 24, 5142-5158.
- Saunders, D.G.O.; Breen, S.; Win, J.; Schornack, S.; Hein, I.; Bozkurt, T.O.; Champouret, N.; Vleeshouwers, G.A.A.; Birch, P.R.J.; Gilroy, E.M.; Kamoun, S. (2012) Host protein BSL1 associates with phytophthora infestans RXLR effector AVR2 and the solanum demissum immune receptor R2 to mediate disease resistance., The Plant Cell, 24, 3420-3434.
- Cooke, D.E.L.; Cano, L.M.; Raffaele, S.; Bain, R.A.; Cooke, L.R.; Etherington, G.J.; Deahl, K.L.; Farrer, R.A.; Gilroy, E.M.; Goss, E.M.; Grunwald, N.J.; Hein, I.; Maclean, D.; McNicol, J.W.; Randall, E.; Oliva, R.F.; Pel, M.A.; Shaw, D.S.; Squires, J.N.; Taylor, M.C.; Vleeshouwers, V.G.A.A.; Birch, P.R.J.; Lees, A.K.; Kamoun, S. (2012) Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen., PLoS Pathogens, 8, Article No. e1002940.
- Jupe, F.; Pritchard, L.; Etherington, G.; MacKenzie, K.; Cock, P.J.A.; Wright, F.; Sharma, S.K.; Bolser, D.; Bryan, G.J.; Jones, J.; Hein, I. (2012) Identification and localisation of the NB-LRR gene family within the potato genome., BMC Genomics,13, 1-14.
- Birch, P.R.J.; Bryan, G.J.; Fenton, B.; Gilroy, E.M.; Hein, I.; Jones, J.T.; Prashar, A.; Taylor, M.A.; Torrance, L.; Toth, I.K. (2012) Crops that feed the world 8: potato: are the trends of increased global production sustainable?, Food Security, 4, 477-508.
- Whisson, S.C.; Avrova, A.O.; Boevink, P.C.; Armstrong, M.R.; Seman, Z.A.; Hein, I.; Birch, P.R.J. (2011) Exploiting knowledge of pathogen effectors to enhance late blight resistance in potato., Potato Research, 54, 325-340.
- Sun, X.L.; Gilroy, E.M.; Chini, A.; Nurmberg, P.L.; Hein, I.; Lacomme, C.; Birch, P.R.J.; Hussain, A.; Yun, B.W.; Loake, G.J. (2011) ADS1 encodes a MATE-transporter that negatively regulates plant disease resistance., New Phytologist, 192, 471-482.
- Gilroy, E.M.; Breen, S.; Whisson, S.C.; Squires, J.; Hein, I.; Kaczmarek, M.; Turnbull, D.; Boevink, P.; Lokossou, A.; Cano, L.M.; Morales, J.; Avrova, A.O.; Pritchard, L.; Randall, E.; Lees, A.K.; Govers, F.; van West, P.; Kamoun, S.; Vleeshouwers, V.G.A.A.; Cooke, D.E.L.; Birch, P.R.J. (2011) Presence/absence, differential expression and sequence polmorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants., New Phytologist, 191, 763-776.
- Gilroy, E.M.; Taylor, R.M.; Hein, I.; Boevink, P.; Sadanandom, A.; Birch, P.R.J. (2011) CMPG1-dependent cell death follows perception of diverse pathogen elicitors at the host plasma membrane and is suppressed by Phytophthora infestans RXLR effector AVR3a., New Phytologist, 190, 653-666.
- Rietman, H.; Champouret, N.; Hein, I.; Niks, R.E.; Vleeshouwers, V.G.A.A. (2010) Plant and oomycetes, an intimate relationship: Co-evolutionary principles and impact on agricultural practice., CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 5, 1-17.
- Bos, J.I.B.; Armstrong, M.R.; Gilroy, E.M.; Boevink, P.; Hein, I.; Taylor, R.M.; Tian, Z.D.; Engelhardt, S.; Vetukuri, R.R.; Harrower, B.E.; Dixelius, C.; Bryan, G.J.; Sadanandom, A.; Whisson, S.C.; Kamoun, S.; Birch, P.R.J. (2010) Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 9909-9914.
- Hein, I.; Birch, P.R.J.; Danan, S.; Lefebvre, V.; Odeny, D.A.; Gebhardt, C.; Trognitz, F.; Bryan, G.J. (2009) Progress in mapping and cloning qualitative and quantitative resistance against Phytophthora infestans in potato and its wild relatives., Potato Research, 52, 215-227.
- Hein, I.; Gilroy, E.M.; Armstrong, M.R.; Birch, P.R.J. (2009) The zig-zag-zig in oomycete-plant interactions., Molecular Plant Pathology, 10, 547-562.
- Crampton, B.G.; Hein, I.; Berger, D.K. (2009) Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)., Molecular Plant Pathology, 10, 291-304.
- Graham, J.; Hackett, C.A.; Smith, K.; Woodhead, M.; Hein, I.; McCallum, S. (2009) Mapping QTLs for developmental traits in raspberry from bud break to ripe fruit., Theoretical and Applied Genetics, 118, 1143-1155.
- Song, B.K.; Hein, I.; Druka, A.; Waugh, R.; Marshall, D.F.; Nadarajah, K.; Yap, S.J.; Ratnam, W. (2009) The 172-kb genomic DNA region of the O. rufipogon ylg1.1 locus: comparative sequence analysis with O. sativa ssp. Japonica and O. sativa ssp. Indica., Functional and Integrative Genomics, 9, 97-108.
- Birch, P.R.J.; Boevink, P.; Gilroy, E.M.; Hein, I.; Pritchard, L.; Whisson, S.C. (2008) Oomycete RXLR effectors: delivery, functional redundancy and durable disease resistance., Current Opinion in Plant Biology, 11, 373-379.
- Sharma, S.K.; Millam, S.; Bryan, G.J.; Hein, I. (2008) Cloning and molecular characterisation of a potato SERK gene transcriptionally induced during initiation of somatic embryogenesis., Planta, 228, 319-330.
- Hein, I.; McLean, K.; Chalhoub, B.; Bryan, G.J. (2007) Generation and screening of a BAC library from a diploid potato clone to unravel durable late blight resistance on linkage group IV., International Journal of Plant Genomics, 2007.
- Whisson, S.C.; Boevink, P.; Moleleki, L.N. ; Avrova, A.O.; Morales, J.G.; Gilroy, E.M.; Armstrong, M.R.; Grouffaud, S.; van West, P.; Chapman, S.N.; Hein, I.; Toth, I.K.; Pritchard, L.; Birch, P.R.J. (2007) A translocation signal for delivery of oomycete effector proteins into host plant cells., Nature, 450, 115-118.
- van den, Berg, N.; Berger, D.K.; Hein, I.; Birch, P.R.J.; Wingfield, M.J.; Viljoen, A. (2007) Tolerance in banana to Fusarium wilt is associated with early up-regulation of cell wall-strengthening genes in the roots., Molecular Plant Pathology, 8, 333-341.
- Gilroy, E.M.; Hein, I.; van der Hoorn, R.; Boevink, P.; Venter, E.; McLellan, H.; Kaffarnik, F.; Hrubikova, K.; Shaw, J.; Holeva, M.C.; Lopez, E.C.; Borras-Hidalgo, O.; Pritchard, L.; Loake, G.J.; Lacomme, C.; Birch, P.R.J. (2007) Involvement of cathepsin B in the plant disease resistance hypersensitive response., Plant Journal, 52, 1-13.
- Avrova, A.O.; Whisson, S.C.; Pritchard, L.; Venter, E.; de Luca, S.; Hein, I.; Birch, P.R.J. (2007) A novel, non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans., Microbiology, 153, 747-759.
- Baker, K.; Stephen, G.; Strachan, S.; Armstrong, M.; Hein, I. (2018) BLASTmap: A Shiny-based application to visualize BLAST results as interactive heat maps and a tool to design gene-specific baits for bespoke target enrichment sequencing., In: Ma, W. & Wolpert, T. (eds.). Plant Pathogenic Fungi and Oomyctes. Methods in Molecular Biology, Humana Press, New York, Volume 1848, pp199-206.
- Feechan, A.; Turnbull, D.; Stevens, L.J.; Engelhardt, S.; Birch, P.R.J.; Hein, I.; Gilroy, E.M. (2015) The hypersensitive response in PAMP- and effector-triggered immune responses., In: Gunawardena, A. & McCabe, P. (eds.). Plant Programmed Cell Death. Springer, New York, Chapter 10, pp235-258.
- Jupe, F.; Chen, X.; Verweij, W.; Witek, K.; Jones, J.D.G.; Hein I. (2014) Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants., In: Birch, P.R.J., Jones, J.T. & Bos, J.I.B. (eds.). Plant-Pathogen Interactions: Methods in Molecular Biology. Volume 1127, Humana Press, New York, 2nd ed, Chapter 22, pp291-303.
- Hein, I.; Kumlehn, J.; Waugh, R. (2008) Functional validation in the Triticeae., In: Jorgensen, R. (ed.). Genetics and Genomics of the Triticeae. Chapter 13, 359-286.
- Graham, J.; Hein, I.; Powell, W. (2007) Raspberry., In: Kole, C (ed.). Genome Mapping and Molecular Breeding in Plants: Fruits and Nuts. Springer, Berlin Heidelberg, Chapter 9, 207-216.
- Graham, J.; Hein, I.; Powell, W. (2007) Genomic tools in contemporary raspberry breeding., In: Kole, C. (ed.). Genome Mapping and Molecular Breeding in Plants. Vol 4 Fruits and Nuts. Springer-Verlag, Berlin, Chapter 9, 207-214.
- Berger, D.K.; Crampton, B.G.; Hein, I.; Vos, W. (2007) Screening cDNA libraries on glass slide microarrays., In: Brampal, J.B. (ed.). Microarrays: Volume II Applications and Data Analysis, Series: Methods in Molecular Biology Vol 382. 2nd edition. Humana Press, Totowa, New Jersey, USA, Chapter 12, 177-203.
- Hein, I.; Birch, P.R.J.; Chapman, S.N.; Bryan, G.J. (2011) Pathogen effector driven search for more durable potato late blight resistance., Annual Report of the Scottish Crop Research Institute for 2010, pp38-40.
- Avrova, A.O.; Birch, P.R.J.; Hein, I.; Jones, J.T.; Pritchard, L. (2010) Functional analysis of pathogen effectors: how to go from genomics to durable resistance., Annual Report of the Scottish Crop Research Institute for 2009, 39-41.
- Sharma, S.K.; Millam, S.; Hedley, P.E.; Hein, I.; McNicol, J.W.; Bryan, G.J. (2008) Somatic embryogenesis in potato., Annual Report of the Scottish Crop Research Institute for 2007, pp18-19.
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