Skip to navigation Skip to content

Ingo Hein

Staff picture: Ingo Hein
Cell and Molecular Sciences
Cell and Molecular Sciences
Senior Research Scientist
Ingo.Hein@hutton.ac.uk
+44 (0)344 928 5428 (*)

The James Hutton Institute
Invergowrie
Dundee DD2 5DA
Scotland UK

 

Current research interests

My research aims to identify and clone more durable resistances towards major potato pathogen.

All microbes trigger immune responses in plants via host receptor-mediated recognition of PAMPs. Adapted pathogens suppress or otherwise manipulate PAMP-triggered immunity (PTI). They do this by secretion of virulence factors, called effectors, which re-programme host metabolism to the benefit of the pathogen.

Effector-triggered suppression of PTI is the pathogen’s front-line of attack in overcoming plant defences. When a successful pathogen has suppressed PTI, plants possess a second layer of inducible defence in the form of resistance (R) genes, the products of which typically encode NB-LRR proteins that detect effectors (termed avirulence proteins; AVRs).

In a “paradigm-shift” from conventional disease resistance breeding we exploit our knowledge of recent dramatic changes in the pathogen population to unravel the underlying variation in pathogen effectors, using this to drive the search for durable resistance.

A central component of our research is the exploitation of the recently sequenced potato genome and use of second generation sequencing and novel DNA capture technology to greatly accelerate discovery and isolation of functional resistance genes.

Current projects include:

  • Identification and cloning of new genetic variations in Solanum species for resistance to Phytophthora infestans.
  • Identification of the Phytophthora infestans effectors underpinning Ph2 and Ph3 resistance in tomato.Identification of potato NB-LRR genes and development of a second generation sequencing approach to clone functional R genes more rapidly
  • Phytophthora infestans effector Avr3a diversity and function.
  • Enhancing the recognitions specificity of known Solanum resistance genes in vitro.

Past research

  • 2010 – present: Principal Investigator potato disease resistance group.  
  • 2006 – 2010: Senior Postdoctoral Researcher (Band 5 since 2008;  SCRI)
    Studying potato disease resistance to Phytophthora infestans.
  • 2003 – 2006: Postdoctoral Researcher (Band 6; SCRI)
    Elucidating disease resistance pathways in potato and barley. Generation of genomic tools for studying root rot resistance in raspberry.
  • March 2003: Doctor of Philosophy
    Thesis title: Discovering Resistance Pathways in Response to Elicitors and Powdery Mildew Attack in Barley; University of Dundee, Department of Life Science, Dundee, United Kingdom
  • October 1999: Diplom Biologe (Master in Biology)
    Thesis title: Functional characterisation of a Homeobox family in Flaveria
    Heinrich-Heine-University Düsseldorf, Institut für Entwicklungs- & Molekularbiologie der Pflanzen, Düsseldorf, Germany

Bibliography

  • Baker, K.; Stephen, G.; Strachan, S.; Armstrong, M.; Hein, I., (2018) BLASTmap: A Shiny-based application to visualize BLAST results as interactive heat maps and a tool to design gene-specific baits for bespoke target enrichment sequencing., In: Ma, W. & Wolpert, T. (eds.). Plant Pathogenic Fungi and Oomyctes. Methods in Molecular Biology, Humana Press, New York, Volume 1848, pp199-206.
  • Feechan, A.; Turnbull, D.; Stevens, L.J.; Engelhardt, S.; Birch, P.R.J.; Hein, I.; Gilroy, E.M., (2015) The hypersensitive response in PAMP- and effector-triggered immune responses., In: Gunawardena, A. & McCabe, P. (eds.). Plant Programmed Cell Death. Springer, New York, Chapter 10, pp235-258.
  • Jupe, F.; Chen, X.; Verweij, W.; Witek, K.; Jones, J.D.G.; Hein I., (2014) Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants., In: Birch, P.R.J., Jones, J.T. & Bos, J.I.B. (eds.). Plant-Pathogen Interactions: Methods in Molecular Biology. Volume 1127, Humana Press, New York, 2nd ed, Chapter 22, pp291-303.
  • Hein, I.; Kumlehn, J.; Waugh, R., (2008) Functional validation in the Triticeae., In: Jorgensen, R. (ed.). Genetics and Genomics of the Triticeae. Chapter 13, 359-286.
  • Graham, J.; Hein, I.; Powell, W., (2007) Raspberry., In: Kole, C (ed.). Genome Mapping and Molecular Breeding in Plants: Fruits and Nuts. Springer, Berlin Heidelberg, Chapter 9, 207-216.
  • Graham, J.; Hein, I.; Powell, W., (2007) Genomic tools in contemporary raspberry breeding., In: Kole, C. (ed.). Genome Mapping and Molecular Breeding in Plants. Vol 4 Fruits and Nuts. Springer-Verlag, Berlin, Chapter 9, 207-214.
  • Berger, D.K.; Crampton, B.G.; Hein, I.; Vos, W., (2007) Screening cDNA libraries on glass slide microarrays., In: Brampal, J.B. (ed.). Microarrays: Volume II Applications and Data Analysis, Series: Methods in Molecular Biology Vol 382. 2nd edition. Humana Press, Totowa, New Jersey, USA, Chapter 12, 177-203.

  • Hein, I.; Birch, P.R.J.; Chapman, S.N.; Bryan, G.J., (2011) Pathogen effector driven search for more durable potato late blight resistance., Annual Report of the Scottish Crop Research Institute for 2010, pp38-40.
  • Avrova, A.O.; Birch, P.R.J.; Hein, I.; Jones, J.T.; Pritchard, L., (2010) Functional analysis of pathogen effectors: how to go from genomics to durable resistance., Annual Report of the Scottish Crop Research Institute for 2009, 39-41.
  • Sharma, S.K.; Millam, S.; Hedley, P.E.; Hein, I.; McNicol, J.W.; Bryan, G.J., (2008) Somatic embryogenesis in potato., Annual Report of the Scottish Crop Research Institute for 2007, pp18-19.

Printed from /staff/ingo-hein on 10/12/18 07:54:29 PM

The James Hutton Research Institute is the result of the merger in April 2011 of MLURI and SCRI. This merger formed a new powerhouse for research into food, land use, and climate change.