Jorunn Bos
Current research interests
My research is aimed at understanding how aphids modify their host plants at the molecular level to enable successful infestations. Aphids are devastating plant sap-feeding insects. These insects cause direct feeding damage and transmit the majority of plant viruses, resulting in significant yield losses, particularly in staple food crops.
Aphid control relies on insecticides that are damaging to the environment and to which aphids can become resistant. To develop new control strategies we need to understand how these insects interact with plants at the cellular and molecular level.
Key questions herein are: Which plant cellular processes are perturbed by aphids and how does that enable infestation? What proteins, and potentially other molecules" do aphids “inject” into host plants to establish successful infestations? Can we use knowlegde on aphid effectors to generate crops with durable resistance?
Aphids, like plant microbial pathogens and nematodes, secrete effectors into their host plants to manipulate host cell processes and impact the ability to infest plants. These effectors are produced in the aphid salivary glands and secreted into the host during aphid feeding. Our lab is interested in understanding the functions of these proteins by identifying their plant targets, characterising their functions in susceptibility, and understanding how effecotr and target protein structures relate to function. We used a wide range of approaches, including structural biology, biochemistry, in planta functional assays for example to assess aphid performance upon over/ectopic expression of effectors and targets.
Our effector biology work mostly focuses on the aphid species Myzus persicae (green peach aphid), a major pest globally, but makes use of comparative genomics data of a wide range of aphid species.
In addition we are interested in identifying novel sources of crop resistance, predominantly in the cereal crop barley.
Past research
Current: Research Leader, Cell and Molecular Sciences, The James Hutton Instittute (50% appointment) and Reader, Division of Plant Sciences (University of Dundee) (50% appointment)
October 2010 - 2016: Independent Research Fellow, The James Hutton Institute - Royal Society of Edinburgh/Scottish Government Marie Curie Personal Research Fellowship 2010-2015. Joint appointment with the Division of Plant Sciences, University of Dundee.
November 2008 - October 2010: Post-doc, The John Innes Centre, Norwich, UK
October 2007 - November 2008: Post-doc, The Sainsbury Laboratory, Norwich, UK
September 2003 - August 2007: PhD, The Ohio State University, Wooster OH, USA
Bibliography
- Leybourne, D.J.; Valentine, T.A.; Binnie, K.; Taylor, A.; Karley, A.J.; Bos, J.I.B. (2022) Drought stress increases the expression of barley defence genes with negative consequences for infesting cereal aphids , Journal of Experimental Botany, 73(7), 2238-2250
- Leybourne, D.J.; Preedy, K.F.; Valentine, T.A.; Bos, J.I.B.; Karley, A.J. (2021) Drought has negative consequences on aphid fitness and plant vigour: Insights from a meta-analysis., Ecology and Evolution, 11, 11915-11929
- Thorpe, P.; Escudero-Martinez, C.M.; Eves-van den Akker, S.; Bos, J.I.B. (2020) Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions., Insect Molecular Biology, 29, 271-282.
- Leybourne, D.J.; Valentine, T.A.; Bos J.I.B.; Karley, A.J. (2020) A fitness cost resulting from Hamiltonella defensa infection is associated with altered probing and feeding behaviour in Rhopalosiphum padi., Journal of Experimental Biology, 223, Article No. jeb207936.
- Leybourne, D.J.; Bos, J.I.B.; Valentine, T.A.; Karley, A.J. (2020) The price of protection: a defensive endosymbiont impairs nymph growth in the bird cherry-oat aphid, Rhopalosiphum padi., Insect Science, 27, 69-85.
- Leybourne, D.L.; Valentine, T.A.; Robertson, J.A.H.; Perez-Fernandez, E.; Main, A.M.; Karley, A.J.; Bos, J.I.B. (2019) Defence gene expression and phloem quality contribute to mesophyll and phloem resistance to aphids in wild barley., Journal of Experimental Botany, 70, 4011-4026.
- Thorpe, P.; Escudero-Martinez, C.M.; Cock, P.J.A.; Eves-van den Akker, S.; Bos, J.I.B. (2018) Shared transcriptional control and disparate gain and loss of aphid parasitism genes., Genome Biology and Evolution, 10, 2716-2733.
- Escudero-Martinez, C.M.; Morris, J.A.; Hedley, P.E.; Bos, J.I.B. (2017) Barley transcriptome analyses upon interaction with different aphid species identify thionins contributing to resistance., Plant Cell and Environment, 40, 2628-2643.
- Thorpe, P.; Cock, P.J.A.; Bos, J. (2016) Comparative transcriptomics and proteomics of three different aphid species identifies core and diverse effector sets., BMC Genomics, 17, Article No. 172.
- Jaouannet, M.; Morris, J.A.; Hedley, P.E.; Bos, J.B. (2015) Characterization of Arabidopsis transcriptional responses to different aphid species reveals genes that contribute to host susceptibility and non-host resistance., PLoS Pathogens, 11, Article No. e1004918.
- Vega-Arreguin, J.C.; Jalloh, A.; Bos, J.I.; Moffett, P. (2014) Recognition of an Avr3a homologue plays a major role in mediating nonhost resistance to Phytophthora capsici in Nicotiana species., Molecular Plant Microbe Interactions, 27, 770-780.
- Rodriguez, P.A.; Stam, R.; Warbroek, T.; Bos, J.I.B. (2014) Mp10 and Mp42 from the aphid species Myzus persicae trigger plant defenses in Nicotiana benthamiana through different activities., Molecular Plant Microbe Interactions, 27, 30-39.
- Jaouannet, M.; Rodriguez, P.A.; Thorpe, P.; Lenoir, C.J.G.; MacLeod, R.; Escudero-Martinez, C.; Bos, J.I.B. (2014) Plant immunity in plant-aphid interactions., Frontiers in Plant Science, 5, Article No. 663.
- Birch, P.; Jones, J.; Bos, J.I. (eds). (2014) Plant-pathogen interactions: methods and protocols., Methods in Molecular Biology, Volume 1127. 2nd edition, Humana Press, New York, 306pp.
- Rodriguez, P.A.; Bos, J.I.B. (2013) Toward understanding the role of aphid effectors in plant infestation., Molecular Plant Microbe Interactions, 26, 25-30.
- Hogenhout, S.A.; Bos, J.I.B. (2011) Effector proteins that modulate plant-insect interactions., Current Opinion in Plant Biology, 14, 422-428.
- Bos, J.I.B.; Hogenhout, S.A. (2011) Effectors in plant-insect interactions., In: Martin, F. & Kamoun, S. (eds.). Effectors in Plant Microbe Interactions. Wiley-Blackwell, pp355-376.
- Bos, J.I.B.; Armstrong, M.R.; Gilroy, E.M.; Boevink, P.; Hein, I.; Taylor, R.M.; Tian, Z.D.; Engelhardt, S.; Vetukuri, R.R.; Harrower, B.E.; Dixelius, C.; Bryan, G.J.; Sadanandom, A.; Whisson, S.C.; Kamoun, S.; Birch, P.R.J. (2010) Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 9909-9914.
- Bollmann, S.R.; Bos, J.I.B.; Bulone, V.; Cai, G.H.; Cakir, C.; Carrington, J.C.; Chawner, M.; Conti, L; Costanzo, S.; Ewan, R.; Fahlgren, N.; Fischbach, M.A.; Fugelstad, J.; Gilroy, E.M.; Gnerre, S.; Green, P.J.; Grenville-Briggs, L.J.; Griffith, J.; Grunwald, N.J.; Horn, K.; Horner, N.R.; Hu, C.H.; Huitema, E.; Jeong, D.H.; Jones, A.M.E.; Jones, J.D.G.; Jones, R.W.; Karlsson, E.K.; Kunjeti, S.G.; Lamour, K.; Liu, Z.Y.; Ma, L.J.; MacLean, D.; Chibucos, M.C.; McDonald, H.; McWalters, J.; Meijer, H.J.G.; Morgan, W.; Morris, P.F.; Munro, C.A.; O'Neill, K.; Ospina-Giraldo, M.; Pinzon, A.; Pritchard, L.; Ramsahoye, B.; Ren, Q.H.; Restrepo, S.; Roy, S.; Sadanandom, A.; Savidor, A.; Schornack, S.; Schwartz, D.C.; Schumann, U.D.; Schwessinger, B.; Seyer, L.; Sharpe, T.; Silvar, C.; Song, J.; Studholme, D.J.; Sykes, S.; Thines, M.; van de Vondervoort, P.J.I.; Phuntumart, V.; Wawra, S.; Weide, R.; Win, J.; Young, C.; Zhou, S.G.; Fry, W.; Meyers, B.C.; van West, P.; Ristaino, J.; Govers, F.; Birch, P.R.J.; Whisson, S.C.; Judelson, H.S.; Nusbaum, C. (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans., Nature, 461, 393-398.
- Birch, P.R.J.; Armstrong, M.R.; Bos, J.; Boevink, P.; Gilroy, E.M.; Taylor, R.M.; Wawra, S.; Pritchard, L.; Conti, L.; Ewan, R.; Whisson, S.C.; van West, P.; Sadanandom, A.; Kamoun, S. (2009) Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans., Journal of Experimental Botany, 60, 1133-1140.
- Gilroy, E.M.; Armstrong, M.R.; Bos, J.; Taylor, R.; Morales, J.; Hein, I.; Douglas, E.; Boevink, P.; Avrova, A.O.; Breen, S.; van West, P.; Pritchard, L.; Whisson, S.C.; Sadanandom, A.; Kamoun, S.; Birch, P.R.J. (2009) Functional analysis of Phytophthora infestans RXLR effectors Avr2 and Avr3a., Fungal Genetics Conference, Asilomar Conference Grounds, Pacific Grove, California, USA, 17-22 March 2009.
- Birch, P.R.J.; Armstrong, M.R.; Gilroy, E.M.; Bos, J.; Taylor, R.M.; Morales, J.G.; Hein, I.; Douglas, E.; Boevink, P.; Breen, S.; Pritchard, L.; Whisson, S.C.; Sadanandom, A.; Kamoun, S. (2009) Localisation and function of Phytophthora infestans RXLR effectors and their host targets., XIV MPMI Conference, Montreal, Canada, 19-23 July 2009.
- Birch, P.R.J.; Whisson, S.C.; Boevink, P.; Armstrong, M.R.; Gilroy, E.M.; Pritchard, L.; Grouffaud, S.; Sandanandom, A.; Taylor, R.; Kamoun, S.; Bos, J.I. (2008) Translocated oomycete effectors that target the plant immune system., Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 150, S176-S177.
- Bos, J.I.B.; Kanneganti, T.; Young, C.; Cakir, C.; Huitema, E.; Win, J.; Armstrong, M.R.; Birch, P.R.J.; Kamoun, S. (2006) The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana., Plant Journal, 48, 165-176.
- Armstrong, M.R.; Whisson, S.C.; Pritchard, L.; Bos, J.I.B.; Venter, E.; Avrova, A.O.; Rehmany, A.P.; Bohme, U.; Brooks, K.; Cherevach, I.; Hamlin, N.; White, B.; Fraser, A.; Lord, A.; Quail, M.A.; Churcher, C.; Hall, N.; Berriman, M.; Huang, S.; Kamoun, S.; Beynon, J.L.; Birch, P.R.J. (2005) An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognised in the host cytoplasm., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 102, 7766-7771.
- Liu, Z.; Bos, J.I.B.; Armstrong, M.R.; Whisson, S.C.; da Cunha, L.; Torto-Alalibo, T.; Win, J.; Avrova, A.O.; Wright, F.; Birch, P.R.J.; Kamoun, S. (2005) Patterns of diversifying selection in the phytotoxin-like scr74 gene family of Phytophthora infestans., Molecular Biology and Evolution, 22, 659-572.
- Bos, J.I.; Armstrong, M.R.; Whisson, S.C.; Torto, T.A.; Ochwo, M.; Birch, P.R.J.; Kamoun, S. (2003) Intraspecific comparative genomics to identify avirulence genes from Phytophthora., New Phytologist, 159, 63-72.
- Leybourne, D.J.; Valentine, T.A.; Binnie, K.; Taylor, A.; Karley, A.J.; Bos, J.I.B. (2022) Drought stress increases the expression of barley defence genes with negative consequences for infesting cereal aphids , Journal of Experimental Botany, 73(7), 2238-2250
- Leybourne, D.J.; Preedy, K.F.; Valentine, T.A.; Bos, J.I.B.; Karley, A.J. (2021) Drought has negative consequences on aphid fitness and plant vigour: Insights from a meta-analysis., Ecology and Evolution, 11, 11915-11929
- Thorpe, P.; Escudero-Martinez, C.M.; Eves-van den Akker, S.; Bos, J.I.B. (2020) Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions., Insect Molecular Biology, 29, 271-282.
- Leybourne, D.J.; Valentine, T.A.; Bos J.I.B.; Karley, A.J. (2020) A fitness cost resulting from Hamiltonella defensa infection is associated with altered probing and feeding behaviour in Rhopalosiphum padi., Journal of Experimental Biology, 223, Article No. jeb207936.
- Leybourne, D.J.; Bos, J.I.B.; Valentine, T.A.; Karley, A.J. (2020) The price of protection: a defensive endosymbiont impairs nymph growth in the bird cherry-oat aphid, Rhopalosiphum padi., Insect Science, 27, 69-85.
- Leybourne, D.L.; Valentine, T.A.; Robertson, J.A.H.; Perez-Fernandez, E.; Main, A.M.; Karley, A.J.; Bos, J.I.B. (2019) Defence gene expression and phloem quality contribute to mesophyll and phloem resistance to aphids in wild barley., Journal of Experimental Botany, 70, 4011-4026.
- Thorpe, P.; Escudero-Martinez, C.M.; Cock, P.J.A.; Eves-van den Akker, S.; Bos, J.I.B. (2018) Shared transcriptional control and disparate gain and loss of aphid parasitism genes., Genome Biology and Evolution, 10, 2716-2733.
- Escudero-Martinez, C.M.; Morris, J.A.; Hedley, P.E.; Bos, J.I.B. (2017) Barley transcriptome analyses upon interaction with different aphid species identify thionins contributing to resistance., Plant Cell and Environment, 40, 2628-2643.
- Thorpe, P.; Cock, P.J.A.; Bos, J. (2016) Comparative transcriptomics and proteomics of three different aphid species identifies core and diverse effector sets., BMC Genomics, 17, Article No. 172.
- Jaouannet, M.; Morris, J.A.; Hedley, P.E.; Bos, J.B. (2015) Characterization of Arabidopsis transcriptional responses to different aphid species reveals genes that contribute to host susceptibility and non-host resistance., PLoS Pathogens, 11, Article No. e1004918.
- Vega-Arreguin, J.C.; Jalloh, A.; Bos, J.I.; Moffett, P. (2014) Recognition of an Avr3a homologue plays a major role in mediating nonhost resistance to Phytophthora capsici in Nicotiana species., Molecular Plant Microbe Interactions, 27, 770-780.
- Rodriguez, P.A.; Stam, R.; Warbroek, T.; Bos, J.I.B. (2014) Mp10 and Mp42 from the aphid species Myzus persicae trigger plant defenses in Nicotiana benthamiana through different activities., Molecular Plant Microbe Interactions, 27, 30-39.
- Jaouannet, M.; Rodriguez, P.A.; Thorpe, P.; Lenoir, C.J.G.; MacLeod, R.; Escudero-Martinez, C.; Bos, J.I.B. (2014) Plant immunity in plant-aphid interactions., Frontiers in Plant Science, 5, Article No. 663.
- Rodriguez, P.A.; Bos, J.I.B. (2013) Toward understanding the role of aphid effectors in plant infestation., Molecular Plant Microbe Interactions, 26, 25-30.
- Hogenhout, S.A.; Bos, J.I.B. (2011) Effector proteins that modulate plant-insect interactions., Current Opinion in Plant Biology, 14, 422-428.
- Bos, J.I.B.; Armstrong, M.R.; Gilroy, E.M.; Boevink, P.; Hein, I.; Taylor, R.M.; Tian, Z.D.; Engelhardt, S.; Vetukuri, R.R.; Harrower, B.E.; Dixelius, C.; Bryan, G.J.; Sadanandom, A.; Whisson, S.C.; Kamoun, S.; Birch, P.R.J. (2010) Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 9909-9914.
- Bollmann, S.R.; Bos, J.I.B.; Bulone, V.; Cai, G.H.; Cakir, C.; Carrington, J.C.; Chawner, M.; Conti, L; Costanzo, S.; Ewan, R.; Fahlgren, N.; Fischbach, M.A.; Fugelstad, J.; Gilroy, E.M.; Gnerre, S.; Green, P.J.; Grenville-Briggs, L.J.; Griffith, J.; Grunwald, N.J.; Horn, K.; Horner, N.R.; Hu, C.H.; Huitema, E.; Jeong, D.H.; Jones, A.M.E.; Jones, J.D.G.; Jones, R.W.; Karlsson, E.K.; Kunjeti, S.G.; Lamour, K.; Liu, Z.Y.; Ma, L.J.; MacLean, D.; Chibucos, M.C.; McDonald, H.; McWalters, J.; Meijer, H.J.G.; Morgan, W.; Morris, P.F.; Munro, C.A.; O'Neill, K.; Ospina-Giraldo, M.; Pinzon, A.; Pritchard, L.; Ramsahoye, B.; Ren, Q.H.; Restrepo, S.; Roy, S.; Sadanandom, A.; Savidor, A.; Schornack, S.; Schwartz, D.C.; Schumann, U.D.; Schwessinger, B.; Seyer, L.; Sharpe, T.; Silvar, C.; Song, J.; Studholme, D.J.; Sykes, S.; Thines, M.; van de Vondervoort, P.J.I.; Phuntumart, V.; Wawra, S.; Weide, R.; Win, J.; Young, C.; Zhou, S.G.; Fry, W.; Meyers, B.C.; van West, P.; Ristaino, J.; Govers, F.; Birch, P.R.J.; Whisson, S.C.; Judelson, H.S.; Nusbaum, C. (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans., Nature, 461, 393-398.
- Birch, P.R.J.; Armstrong, M.R.; Bos, J.; Boevink, P.; Gilroy, E.M.; Taylor, R.M.; Wawra, S.; Pritchard, L.; Conti, L.; Ewan, R.; Whisson, S.C.; van West, P.; Sadanandom, A.; Kamoun, S. (2009) Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans., Journal of Experimental Botany, 60, 1133-1140.
- Birch, P.R.J.; Whisson, S.C.; Boevink, P.; Armstrong, M.R.; Gilroy, E.M.; Pritchard, L.; Grouffaud, S.; Sandanandom, A.; Taylor, R.; Kamoun, S.; Bos, J.I. (2008) Translocated oomycete effectors that target the plant immune system., Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 150, S176-S177.
- Bos, J.I.B.; Kanneganti, T.; Young, C.; Cakir, C.; Huitema, E.; Win, J.; Armstrong, M.R.; Birch, P.R.J.; Kamoun, S. (2006) The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana., Plant Journal, 48, 165-176.
- Armstrong, M.R.; Whisson, S.C.; Pritchard, L.; Bos, J.I.B.; Venter, E.; Avrova, A.O.; Rehmany, A.P.; Bohme, U.; Brooks, K.; Cherevach, I.; Hamlin, N.; White, B.; Fraser, A.; Lord, A.; Quail, M.A.; Churcher, C.; Hall, N.; Berriman, M.; Huang, S.; Kamoun, S.; Beynon, J.L.; Birch, P.R.J. (2005) An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognised in the host cytoplasm., Proceedings of the National Academy of Sciences of the United States of America (PNAS), 102, 7766-7771.
- Liu, Z.; Bos, J.I.B.; Armstrong, M.R.; Whisson, S.C.; da Cunha, L.; Torto-Alalibo, T.; Win, J.; Avrova, A.O.; Wright, F.; Birch, P.R.J.; Kamoun, S. (2005) Patterns of diversifying selection in the phytotoxin-like scr74 gene family of Phytophthora infestans., Molecular Biology and Evolution, 22, 659-572.
- Bos, J.I.; Armstrong, M.R.; Whisson, S.C.; Torto, T.A.; Ochwo, M.; Birch, P.R.J.; Kamoun, S. (2003) Intraspecific comparative genomics to identify avirulence genes from Phytophthora., New Phytologist, 159, 63-72.
- Birch, P.; Jones, J.; Bos, J.I. (eds). (2014) Plant-pathogen interactions: methods and protocols., Methods in Molecular Biology, Volume 1127. 2nd edition, Humana Press, New York, 306pp.
- Bos, J.I.B.; Hogenhout, S.A. (2011) Effectors in plant-insect interactions., In: Martin, F. & Kamoun, S. (eds.). Effectors in Plant Microbe Interactions. Wiley-Blackwell, pp355-376.
- Gilroy, E.M.; Armstrong, M.R.; Bos, J.; Taylor, R.; Morales, J.; Hein, I.; Douglas, E.; Boevink, P.; Avrova, A.O.; Breen, S.; van West, P.; Pritchard, L.; Whisson, S.C.; Sadanandom, A.; Kamoun, S.; Birch, P.R.J. (2009) Functional analysis of Phytophthora infestans RXLR effectors Avr2 and Avr3a., Fungal Genetics Conference, Asilomar Conference Grounds, Pacific Grove, California, USA, 17-22 March 2009.
- Birch, P.R.J.; Armstrong, M.R.; Gilroy, E.M.; Bos, J.; Taylor, R.M.; Morales, J.G.; Hein, I.; Douglas, E.; Boevink, P.; Breen, S.; Pritchard, L.; Whisson, S.C.; Sadanandom, A.; Kamoun, S. (2009) Localisation and function of Phytophthora infestans RXLR effectors and their host targets., XIV MPMI Conference, Montreal, Canada, 19-23 July 2009.
- Escudero-Martinez, C.; Leybourne, D.; Barakate, A.; Morris, J.; Hedley, P.; Stephens, J.; Bos, J. (2018) Understanding the basis of host and non-host defences during barley-aphid interactions., Phytopathology, 108, S1.247. Abstract.
- Armstrong, M.R.; Whisson, S.C.; Pritchard, L.; Bos, J.I.B.; Venter, E.; Avrova, A.O.; Rehmany, A.P.; Huang, S.; Kamoun, S.; Beynon, J.; Birch, P.R.J. (2005) An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognised in the host cell cytoplasm., Molecular Plant Microbe Interactions Conference, Merida, Mexico, 14-19 December 2005 (Poster).
