Research
Metabolomics technologies collect vast quantities of data which must be processed in a suitable manner for the application. We have developed rigorous tests to ensure the validity of our methods and data analysis techniques to ensure robust data sets.
Publications
The following Publications have not yet been migrated to the James Hutton Institute's Pure service and relate to the research outputs from the two legacy organisations: The Macaulay Land Use Research Institute and The Scottish Crop Research Institute.
Journals
- McDougall, G.J.; Martinussen, I.; Junttila, O.; Verrall, S.R.; Stewart, D. (2011) Assessing the influence of genotype and temperature on polyphenol composition in cloudberry (Rubus chamaemorus L) using a novel mass spectrometric method., Journal of Agricultural and Food Chemistry, 59, 10860-10868.
- Ross, H.A.; Morris, W.L.; Ducreux, L.J.M.; Hancock, R.D.; Verrall, S.R.; Morris, J.A.; Tucker, G.A.; Stewart, D.; Hedley, P.E.; McDougall, G.J.; Taylor, M.A. (2011) Pectin engineering to modify product quality in potato., Plant Biotechnology Journal, 9, 848-856.
- Morris, W.L.; Shepherd, T.; Verrall, S.R.; McNicol, J.W.; Taylor, M.A. (2010) Relationships between volatile and non-volatile metabolites and attributes of processed potato flavour., Phytochemistry, 71, pp. 1765-1773.
- Ross, H.A.; McDougall, G.J.; Vincent, J.F.V.; Stewart, D.; Verrall, S.R.; Taylor, M.A. (2010) Discerning intra-tuber differences in textural properties in cooked Solanum tuberosum group Tuberosum and group Phureja tubers., Journal of the Science of Food and Agriculture, 90, 1527-1832.
- Stushnoff, C.; Ducreux, L.J.M.; Hancock, R.D.; Hedley, P.E.; Holm, D.G.; McDougall, G.J.; McNicol, J.W.; Morris, J.; Morris, W.L.; Sungurtas, J.; Verrall, S.R.; Zuber, T.; Taylor, M.A. (2010) Flavonoid profiling and transcriptome analysis reveals new gene-metabolite correlations in tubers of Solanum tuberosum L., Journal of Experimental Botany, 61, 1225-1238.
- Dobson, G.; Shepherd, T.; Verrall, S.R.; Griffiths, D.W.; Ramsay, G.; McNicol, J.W.; Davies, H.V.; Stewart, D. (2010) A metabolomics study of cultivated potato (Solanum tuberosum) groups, Andigena, Phureja, Stenotomum and Tuberosum using gas chromatography-mass spectrometry., Journal of Agricultural and Food Chemistry, 58, 1214-1223.
- Dobson, G.; Shepherd, T.; Verrall, S.R.; Conner, S.; McNicol, J.W.; Ramsay, G.; Shepherd, L.V.T.; Davies, H.V.; Stewart, D. (2008) Phytochemical diversity in tubers of potato cultivars and landraces using a GC-MS metabolomics approach., Journal of Agricultural and Food Chemistry, 56, 10280-10291.
- Shepherd, T.; Dobson, G.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; McNicol, J.W.; Davies, H.V.; Stewart, D. (2007) Potato metabolomics by GC-MS: what are the limiting factors?., Metabolomics, 3, 475-488.
- Stewart, D.; McDougall, G.J.; Sungurtas, J.; Verrall, S.R.; Graham, J.; Martinussen, I. (2007) Metabolomic approach to identifying bioactive compounds in berries: Advances toward fruit nutritional enhancement., Molecular Nutrition and Food Research, 51, 645-651.
- Nuopponen, M.; Shepherd, T.; Birch, G.M.; Verrall, S.R.; Stewart, D. (2006) Hemicelluloses and pectins in 25 tropical hardwoods., Cellulose Chemistry and Technology, 40, 735-738.
- Bruce, A.; Verrall, S.R.; Hackett, C.A.; Wheatley, R.E. (2004) Identification of volatile organic compounds (VOCs) from bacteria and yeast causing growth inhibition of sapstain fungi., Holzforschung, 58, 193-198.
- Bruce, A.; Stewart, D.D.; Verrall, S.R.; Wheatley, R.E. (2003) Effects of volatiles from bacteria and yeast on the growth and pigmentation of sapstain fungi., International Biodeterioration and Degradation, 51, 101-108.
- Bell, K.S.; Roberts, J.; Verrall, S.R.; Cullen, D.W.; Williams, N.A.; Harrison, J.G.; Toth, I.K.; Cooke, D.E.L.; Duncan, J.M.; Claxton, J.R. (2000) Detection and quantification of Spongospora subterranea f.sp. subterranea in soils and on tubers using specific PCR primers., European Journal of Plant Pathology, 105, 905-915.
Books / chapters
- Dobson, G.; Shepherd, T.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; McNicol, J.W.; Stewart, D.; Davies, H.V. (2007) Application of metabolite and flavour volatile profiling to studies of biodiversity in Solanum species., In: Nikolau, B.L. & Wurtele, E.S. (eds.). Concepts in Plant Metabolomics. Springer, Dordrecht, 259-268. ISBN: 978-1-4020-5607-9
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; Stewart, D.; Davies, H.V. (2007) Profiling of metabolites and volatile flavour compounds from Solanum species using gas chromatography-mass spectrometry., In: Nikolau, B.L. & Wurtele, E.S. (eds.). Concepts in Plant Metabolomics. Springer, Dordrecht, 208-219. ISBN: 978-1-4020-5607-9
Technical / contract reports
- Ross, H.A.; Morris, W.L.; Ducreux, L.J.M.; Hancock, R.D.; Verrall, S.R.; Morris, J.A.; Stewart, D.; Hedley, P.E.; McDougall, G.J.; Taylor, M.A. (2011) The role of pectin methyl esterase in determining potato tuber textural quality., Annual Report of the Scottish Crop Research Institute for 2010, pp32-33.
- McNicol, J.W.; Verrall, S.R.; Shepherd, T.; Dobson, G.; Griffiths, D.W.; Ramsay, G.; Davies, H.V.; Stewart, D. (2006) Statistical analysis of metabolomic data., Annual Report of the Scottish Crop Research Institute for 2006, pp54-55.
Conference papers
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; McNicol, J.W.; Davies, H.V.; Stewart, D. (2006) Use and limitations of GC-MS based metabolite profiling methods for the analysis of metabolite content in freeze-dried potato tubers., 4th International Plant Metabolomics Conference, Reading, 7-10 April 2007. Metabolomics, 2(4), p279.
- Stewart, D.; Davies, H.V.; Verrall, S.R.; Shepherd, T.; Dobson, G.; Marshall, R.; Colquhoun, I.; Le Gall, G. (2004) Metabolic profiling: Platform technologies to detect unintended effects following genetic modification of potato (Solanum tuberosum)., 3rd International Congress on Plant Metabolomics, Iowa, USA, 3-6 June 2004.
- Dobson, G.; Shepherd, T.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; McNicol, J.W.; Stewart, D.; Davies, H.V. (2004) Application of metabolite and flavour volatile profiling to studies of biodiversity in Solanum species., 3rd International Congress on Plant Metabolomics, Iowa, USA, 3-6 June 2004.
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; Stewart, D.; Davies, H.V. (2004) Profiling of metabolites and volatile flavour compounds from Solanum species using gas chromatography-mass spectrometry., 3rd International Congress on Plant Metabolomics, Iowa, USA, 3-6 June 2004.
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; McNicol, J.W.; Stewart, D.; Davies, H.V. (2004) Application of metabolite profiling to studies of biodiversity in Solanum species., Society for Experimental Biology Annual Meetin,. Heriot-Watt Unviersity, 29 March – 2 April 2004.
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Shepherd, L.V.T.; McNicol, J.W.; Deighton, N.; Stewart, D.; Davies, H.V. (2003) Can metabolite profiling be used to identify unintended effects in transgenic tubers?, 16th International Mass Spectrometry Conference, Edinburgh, 31 August – 5 September 2003.
- Squires, J.N.; Daniell, T.J.; Clegg, C.D.; Griffiths, B.S.; Ritz, K.; Verrall, S.R.; Wheatley, R.E. (2001) Effects of plants on diversity of nitrite-reducing bacteria in an upland grassland., Molecular Microbial Ecology Group, York.
- Daniell, T.J.; Clegg, C.D.; Griffiths, B.S.; Ritz, K.; Squires, J.; Verrall, S.R.; Wheatley, R.E. (2001) Coupling between plants and diversity of nitrite-reducing bacteria in an upland pasture., Interactions in the Microbial World: 9th International Symposium on Microbial Ecology, Amsterdam, 26-31 August 2001.
- Bruce, A.; Wheatley, R.E.; Verrall, S.R. (2000) Effect of volatiles from bacteria and yeast on the growth and pigmentation of sap-stain fungi., Proceedings of 31st Meeting of International Research Group on Wood Preservation, Hawaii, 14-19 May 2000.
Conference posters / abstracts
- Ross, H.A.; Gordon, S.; McDougall, G.J.; Verrall, S.R.; Wright, K.M.; Cross, J.V.; Berrie, A.; Xu, X.; Brennan, R.M. (2011) Exploring variation in textural properties in blackcurrant germplasm., Xth International Rubus and Ribes Conference, Zlatibor, Serbia, 22-26 June 2011. (Poster)
- Abreu, I.N.; Kanichukattu, E.N.; Sungurtas, J.; Verrall, S.R.; Stewart, D. (2010) Metabolomics as a route to fruit flavour and functionality: blackcurrant as a model study., 28th International Horticultural Congress, Lisbon, Portugal, 22-27 August 2010 (Poster and Talk).
- Verrall, S.R.; McNicol, J.W.; Shepherd, T.; Dobson, G.; Griffiths, D.W.; Ramsay, G.; Stewart, D. (2007) Using hierarchical clustering of metabolomic data to relate clusters to metabolite pathways., 3rd International Metabolomics Society Conference, Manchester, 10-14 June 2007.
- Dobson, G.; Shepherd, T.; Griffiths, D.W.; Ross, H.A.; Verrall, S.R.; Stewart, D. (2007) Studies of tuber developmental stages in the potato Solanum tuberosum group phureja using GC-MS-based metabolomics and volatile analysis approaches., 3rd International Metabolomics Conference. Manchester, 10-14 June 2007.
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; Stewart, D.; Davies, H.V. (2004) Use of gas chromatography-(time-of-flight) mass spectrometry for analysis of metabolites from Solanum species., Society for Experimental Biology Annual Meeting, Heriot Watt University, 29 March – 2 April 2004.
- Verrall, S.R.; Deighton, N.; Marshall, R.; Sungurtas, J.; Shepherd, T.; Shepherd, L.V.T.; Dobson, G.; McNicol, J.W.; Davies, H.V. (2003) Can metabolomics identify unintended effects in transgenic tubers?, 2nd International Conference on Plant Metabolomics, Potsdam, Germany, 25-28 April 2003. (Poster)
- Shepherd, T.; Dobson, G.; Marshall, R.; Verrall, S.R.; Conner, S.; Griffiths, D.W.; Stewart, D.; Davies, H.V. (2003) Analysis of metabolites from Solanum species using gas chromatography-(Time-of-Flight) mass spectrometry and automated data analysis., 16th International Mass Spectrometry Conference, Edinburgh, 31 August – 5 September 2003. (Poster)